Academic Journal
Immunosuppression by Moloney Leukemia Virus: Lack of Correlation Between Virus Replication and the Immunosuppressive Effect
| العنوان: | Immunosuppression by Moloney Leukemia Virus: Lack of Correlation Between Virus Replication and the Immunosuppressive Effect |
|---|---|
| المؤلفون: | Cerny, J., Proffitt, M. R., Essex, M. |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 1976 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Clinical and Laboratory Investigations on Animals |
| الوصف: | Young adult mice were infected with 104 plaque-forming units (PFU) of Moloney murine leukemia virus M-MuLV. Two different virus preparations were used: a ) M-MuLV obtained from serial passage in mice [animal passage (AP)] and b ) tissue culture (TC)-grown virus harvested after three in vitro passages of the AP M-MuLV in fibroblasts. Replication of TC and AP M-MuLV in spleen cells was determined by an infectious center (IC) assay at 1 and 2 weeks after the infection. Immune responsiveness of spleen cells was evaluated in challenge with sheep red blood cells (SRBC) and subsequent enumeration of antibody plaque-forming cells (PFC). TC M-MuLV replicated faster in the spleen than did AP M-MuLV and reached about 10- to 100-fold higher titers. However, the response of anti-SRBC PFC, suppressed to the same degree in the spleens of mice infected with TC or AP virus, was from 10 to 50% of the control response. A comparison of virus replication with the anti-SRBC response in aliquots from the same spleens showed no correlation between virus IC and antibody PFC. Both TC and AP M-MuLV induced the expression of virus-specific, cell membrane antigen on spleen cells. These findings indicated a divergence between virus replication on the one hand and the immunosuppressive effect and the cell membrane alteration on the other. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://jnci.oxfordjournals.org/cgi/content/short/56/4/819; http://dx.doi.org/10.1093/jnci/56.4.819 |
| DOI: | 10.1093/jnci/56.4.819 |
| الاتاحة: | http://jnci.oxfordjournals.org/cgi/content/short/56/4/819 https://doi.org/10.1093/jnci/56.4.819 |
| Rights: | Copyright (C) 1976, National Cancer Institute |
| رقم الانضمام: | edsbas.A414B258 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/jnci/56.4.819 |
|---|