التفاصيل البيبلوغرافية
| العنوان: |
Insight into ortho-boronoaldehyde conjugation via a FRET-based reporter assay |
| المؤلفون: |
Rose, Nicholas, Tipple, Eve, Sanchez, Anais, Lynam, Jason, Spicer, Christopher |
| بيانات النشر: |
American Chemical Society (ACS) |
| سنة النشر: |
2022 |
| الوصف: |
Ortho-boronoaldehydes react with amine-based nucleophiles with dramatically increased rates and product stabilities, relative to unfunctionalised benzaldehydes, leading to exciting applications across biological and material chemistry. We have developed a novel Förster resonance energy transfer (FRET)-based assay to provide key new insights into the reactivity of these boronoaldehydes, allowing us to track conjugation with unprecedented sensitivity and accuracy under standardised conditions. Our results highlight the key role played by reaction pH, buffer additives, and boronoaldehyde structure in controlling conjugation speed and stability, providing design criteria for further innovations and applications in the field. |
| نوع الوثيقة: |
other/unknown material |
| اللغة: |
unknown |
| DOI: |
10.26434/chemrxiv-2022-l269j |
| الاتاحة: |
http://dx.doi.org/10.26434/chemrxiv-2022-l269j
https://chemrxiv.org/engage/api-gateway/chemrxiv/assets/orp/resource/item/62f6a05818037306a7a35c4f/original/insight-into-ortho-boronoaldehyde-conjugation-via-a-fret-based-reporter-assay.pdf |
| Rights: |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.A40630A9 |
| قاعدة البيانات: |
BASE |