Academic Journal
The effect of epidermal growth factor and IGF-I infusion on hepatic and renal expression of the IGF-system in adult female rats
| العنوان: | The effect of epidermal growth factor and IGF-I infusion on hepatic and renal expression of the IGF-system in adult female rats |
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| المؤلفون: | van Neck, JW, Berghout, EM, Vinter-Jensen, L, Groffen, CA, Cingel, V, Dits, NF, Drop, SL, Flyvbjerg, A |
| المصدر: | Journal of Endocrinology ; volume 165, issue 1, page 115-122 ; ISSN 0022-0795 1479-6805 |
| بيانات النشر: | Bioscientifica |
| سنة النشر: | 2000 |
| الوصف: | Systemic administration of epidermal growth factor (EGF) in neonatal rats results in reduced body weight gain and decreased circulating levels of IGF-I, suggesting its involvement in EGF-induced growth retardation. We investigated the effect of EGF and/or IGF-I administration for 7 days on circulating IGF-I and IGFBP levels and hepatic and renal IGF-system mRNA expression profiles in adult female rats. EGF administration (30 microg/rat/day) did not influence body weight, liver or kidney weight. In contrast, IGF-I (400 microg/rat/day) and EGF/IGF-I administration increased both body weight and kidney weight. Also, serum IGF-I and the 30 kDa IGFBPs (IGFBP-1 and -2) were significantly increased in these groups. Serum IGFBP-3 levels increased in the IGF-I group along with increased hepatic IGFBP-1 and -3 mRNA levels. In contrast, in the EGF administration group serum IGFBP-3 levels were significantly decreased; however, the mRNA levels remained unchanged. In the EGF/IGF-I administration group, serum IGF-I and IGFBP-3 levels were significantly lowered when compared with the IGF-I administration group. This was in contrast to the effect on kidney weight increase that was identical for the IGF-I and EGF/IGF-I groups. The decrease in serum IGFBP-3 was not reflected at the hepatic IGFBP-3 mRNA level. IGFBP-3 expression might be regulated at a post-transcriptional level although EGF induced IGFBP-3 proteolysis could not be demonstrated in vitro. We conclude that EGF administration reduced serum IGFBP-3 whereas IGF-I administration increased the level of IGFBP-3 and IGF-I and resulted in an increased body and kidney weight in adult female rats. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| DOI: | 10.1677/joe.0.1650115 |
| الاتاحة: | http://dx.doi.org/10.1677/joe.0.1650115 https://joe.bioscientifica.com/view/journals/joe/165/1/115.xml https://joe.bioscientifica.com/downloadpdf/journals/joe/165/1/115.xml |
| رقم الانضمام: | edsbas.A3EC24C7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1677/joe.0.1650115 |
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