Academic Journal
Hemocyte-targeted gene expression in the female malaria mosquito using the hemolectin promoter from Drosophila
| العنوان: | Hemocyte-targeted gene expression in the female malaria mosquito using the hemolectin promoter from Drosophila |
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| المؤلفون: | Pondeville, Emilie, Puchot, Nicolas, Parvy, Jean-Philippe, Carissimo, Guillaume, Poidevin, Mickael, Waterhouse, Robert M., Marois, Eric, Bourgouin, Catherine |
| المساهمون: | Génomique évolutive, modélisation et santé (GEMS), Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Centre de génétique moléculaire (CGM), Centre National de la Recherche Scientifique (CNRS), Swiss Institute of Bioinformatics Lausanne (SIB), Université de Lausanne = University of Lausanne (UNIL), Modèles Insectes de l'Immunité Innée (M3I), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Support to E.P. was from an ANR-07-MIME-O25-01 award from the French Agence Nationale de la Recherche to C.B., from Fondation Roux (Institut Pasteur) fellowship and by the UK Medical Research Council to E.P. (MC_UU_12014/8), to C.B. from ANR-07-MIME-O25-01 award from the French Agence Nationale de la Recherche to C.B. and ANR-10-LABX-62-IBEID. R.M.W. was supported by Swiss National Science Foundation grant PP00P3_170664., ANR-07-MIME-0025,TRANSANO,Développement d'une méthode nouvelle et efficace de transgénèse chez Anopheles gambiae pour l'étude fonctionnelle des interactions Plasmodium falciparum-Anophèles(2007), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010) |
| المصدر: | ISSN: 0965-1748 ; Insect Biochemistry and Molecular Biology ; https://hal.science/hal-02571412 ; Insect Biochemistry and Molecular Biology, 2020, 120, pp.103339. ⟨10.1016/j.ibmb.2020.103339⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Hemolectin, Immunity, Melanization, Mosquito, onyong-nyong virus, rna interference, Hemocytes, infection, system, phagocytosis, activation, africa, Anopheles, anopheles-gambiae, Arbovirus, complement-like protein, Gal4-UAS system, Granulocytes, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity |
| الوصف: | Place: Oxford Publisher: Pergamon-Elsevier Science Ltd WOS:000525825800004 ; International audience ; Hemocytes, the immune cells in mosquitoes, participate in immune defenses against pathogens including malaria parasites. Mosquito hemocytes can also be infected by arthropod-borne viruses but the pro- or anti-viral nature of this interaction is unknown. Although there has been progress on hemocyte characterization during pathogen infection in mosquitoes, the specific contribution of hemocytes to immune responses and the hemocyte-specific functions of immune genes and pathways remain unresolved due to the lack of genetic tools to manipulate gene expression in these cells specifically. Here, we used the Gal4-UAS system to characterize the activity of the Drosophila hemocyte-specific hemolectin promoter in the adults of Anopheles gambiae, the malaria mosquito. We established an hml-Gal4 driver line that we further crossed to a fluorescent UAS responder line, and examined the expression pattern in the adult progeny driven by the hml promoter. We show that the hml regulatory region drives hemocyte-specific transgene expression in a subset of hemocytes, and that transgene expression is triggered after a blood meal. The hml promoter drives transgene expression in differentiating prohemocytes as well as in differentiated granulocytes. Analysis of different immune markers in hemocytes in which the hml promoter drives transgene expression revealed that this regulatory region could be used to study phagocytosis as well as melanization. Finally, the hml promoter drives transgene expression in hemocytes in which o'nyong-nyong virus replicates. Altogether, the Drosophila hml promoter constitutes a good tool to drive transgene expression in hemocyte only and to analyze the function of these cells and the genes they express during pathogen infection in Anopheles gambiae. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/32105779; hal-02571412; https://hal.science/hal-02571412; https://hal.science/hal-02571412/document; https://hal.science/hal-02571412/file/1-s2.0-S096517482030028X-main.pdf; PUBMED: 32105779; PUBMEDCENTRAL: PMC7181189 |
| DOI: | 10.1016/j.ibmb.2020.103339 |
| الاتاحة: | https://hal.science/hal-02571412 https://hal.science/hal-02571412/document https://hal.science/hal-02571412/file/1-s2.0-S096517482030028X-main.pdf https://doi.org/10.1016/j.ibmb.2020.103339 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.A3A0C44D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ibmb.2020.103339 |
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