Academic Journal
Cornus Officinalis Significantly Improves Oxidative Capacity and Promotes the Calcium-Dependent Transcription Factor, NFATC2, in Human 1.1B4 Pancreatic Cell Line
| العنوان: | Cornus Officinalis Significantly Improves Oxidative Capacity and Promotes the Calcium-Dependent Transcription Factor, NFATC2, in Human 1.1B4 Pancreatic Cell Line |
|---|---|
| المؤلفون: | Sharp, Arielle, Coiner, Alexis, Burkhardt, Brant |
| المصدر: | Cell Biology, Microbiology, and Molecular Biology Faculty Publications |
| بيانات النشر: | Digital Commons @ University of South Florida |
| سنة النشر: | 2019 |
| المجموعة: | University of South Florida St. Petersburg: Digital USFSP |
| مصطلحات موضوعية: | Cell and Developmental Biology, Cell Biology, Life Sciences, Microbiology, Molecular Biology |
| الوصف: | Type 1 diabetes (T1D) is an autoimmune disease resulting in the destruction of pancreatic β cells (β-cells) and subsequent loss of insulin production. The only treatment for T1D is using exogenous insulin coupled with continual glucose monitoring following significant autoimmune destruction of β-cells. Novel interventional therapies are needed that can preserve and protect existing pancreatic β cells in individuals with early identified T1D autoimmunity. Our initial in-vitro evidence indicates Cornus officinalis (CO) may be able to serve in this function. What sets ethnopharmacology apart from conventional medicine is the simultaneous targeting of multiple mechanisms using a single herb due to the composition of numerous bioactive ingredients. CO has been used in TCM (traditional Chinese medicine) for over 2 millennia for the therapeutic effect of improved glucose tolerance and has also demonstrated efficacy in animal models but rarely examined in the context of T1D. We hypothesize that CO treatment may provide a β cell restorative and protective therapy for T1D and inhibit progressive cytokine-mediated β cell loss while enhancing existing β cells. Our preliminary evidence demonstrated a dose-dependent exposure of 1.1B4 cells to CO increased proliferation and protected against cytokine-induced cell death. We examined the metabolic effect of CO using the Agilent Seahorse XF Analyzer and at a two-hour time point there was a remarkable increase in maximal respiratory and glycolytic capacity following CO treatment. However, the molecular mechanism in which CO is inducing a proliferative and metabolic effect in 1.1B4 cells has not been elucidated. Therefore, we employed transcriptomics by RNA-Seq to analyze the early initiators of this increased metabolic effect. Our strongest and most significantly differentially expressed transcript was calcium-dependent transcription factor, NFATc. Expression of NFATc was validated by qPCR, which displayed a 2-fold increase in gene expression. NFATc is an essential transcription ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | https://digitalcommons.usf.edu/bcm_facpub/75; https://digitalcommons.usf.edu/context/bcm_facpub/article/1117/viewcontent/SAT_164_Cornus_Officinalis_Significantly_Improves_Oxidative_Capacity_and_Promotes_the_Calcium_Dependent_Transcription_Factor__NFATC2__in_Human_1.1B4_Pancreatic_Cell_Line___Journal_of_the_Endocrine_Society___Oxford_Academic.pdf |
| DOI: | 10.1210/js.2019-SAT-164 |
| الاتاحة: | https://digitalcommons.usf.edu/bcm_facpub/75 https://doi.org/10.1210/js.2019-SAT-164 https://digitalcommons.usf.edu/context/bcm_facpub/article/1117/viewcontent/SAT_164_Cornus_Officinalis_Significantly_Improves_Oxidative_Capacity_and_Promotes_the_Calcium_Dependent_Transcription_Factor__NFATC2__in_Human_1.1B4_Pancreatic_Cell_Line___Journal_of_the_Endocrine_Society___Oxford_Academic.pdf |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.A393596D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1210/js.2019-SAT-164 |
|---|