Academic Journal
Reduced miR-371b-5p expression drives tumor progression via CSDE1/RAC1 regulation in triple-negative breast cancer
| العنوان: | Reduced miR-371b-5p expression drives tumor progression via CSDE1/RAC1 regulation in triple-negative breast cancer |
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| المؤلفون: | Kim, Yesol, Ko, Je Yeong, Lee, Soo-Been, Oh, Sumin, Park, Jee Won, Kang, Hyeok-Gu, Kim, Da-Hyun, Chung, Daeun, Lim, Sera, Kong, Hyunkyung, Kim, Jongmin, Yoo, Kyung Hyun, Han, Wonshik, Chun, Kyung-Hee, Park, Jong Hoon |
| المساهمون: | National Research Foundation of Korea |
| المصدر: | Oncogene ; volume 41, issue 22, page 3151-3161 ; ISSN 0950-9232 1476-5594 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| الوصف: | Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer; however, specific prognostic biomarkers have not yet been developed. In this study, we identified dysregulated microRNAs (miRNAs) in TNBC by profiling miRNA and mRNA expression. In patients with TNBC, miR-371b-5p expression was reduced, and miR-371b-5p overexpression significantly mitigated TNBC cell growth, migration, and invasion. In addition, we found that expression of cold shock domain-containing protein E1 (CSDE1), a direct target gene of miR-371b-5p, was upregulated in TNBC cells, and inhibition of CSDE1 expression alleviated TNBC cell growth by regulating RAC1 transcription. Mechanistically, CSDE1, phosphorylated C-terminal domain (p-CTD) of RNA polymerase II (RNAPII), and CDK7 form a complex, and downregulation of CSDE1 leads to weak interaction between RNAPII p-CTD and CDK7, resulting in a decrease in RNAPII p-CTD expression to reduce RAC1 transcript levels in CSDE1-deficient TNBC cells. Our data demonstrate that miR-371b-5p is a tumor-suppressive miRNA that regulates the CSDE1/Rac1 axis and could be a potential prognostic biomarker for TNBC. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41388-022-02326-6 |
| الاتاحة: | http://dx.doi.org/10.1038/s41388-022-02326-6 https://www.nature.com/articles/s41388-022-02326-6.pdf https://www.nature.com/articles/s41388-022-02326-6 |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.A39233EA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41388-022-02326-6 |
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