التفاصيل البيبلوغرافية
| العنوان: |
How PQC inhibition modulates miRNA loading in large and small extracellular vesicles |
| المؤلفون: |
E. Casarotto, M. Garofalo, L. Messa, D. Sproviero, S. Carelli, M. Cozzi, M. Chierichetti, R. Cristofani, V. Ferrari, M. Galbiati, F. Mina, M. Piccolella, P. Rusmini, B. Tedesco, P. Pramaggiore, C. Cereda, S. Gagliardi, A. Poletti, V. Crippa |
| المساهمون: |
E. Casarotto, M. Garofalo, L. Messa, D. Sproviero, S. Carelli, M. Cozzi, M. Chierichetti, R. Cristofani, V. Ferrari, M. Galbiati, F. Mina, M. Piccolella, P. Rusmini, B. Tedesco, P. Pramaggiore, C. Cereda, S. Gagliardi, A. Poletti, V. Crippa |
| سنة النشر: |
2022 |
| المجموعة: |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: |
Settore BIO/13 - Biologia Applicata |
| الوصف: |
Extracellular vesicles (EVs) are membrane-enclosed particles released from all eukaryotic cells that carry proteins, lipids, RNA and DNA. They are classified in two main types of EVs: large vesicles (LVs) and small vesicles (SVs). In our previous studies we observed that both LVs and SVs play a role in the disposal of neurotoxic aggregates of the TAR DNA-binding protein 43 (TDP-43) and its C-terminal fragments of 35 (TDP-35) and 25 KDa (TDP-25), associated with Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD).This release increased when the protein quality control (PQC) system [i.e. chaperone proteins, the ubiquitin proteasome system (UPS) and the autophagic pathway] was impaired, a common condition observed both in ALS and FTD. Since TDP-43 is an RNA-binding protein and it is involved in miRNA biogenesis, we wondered whether PQC impairment could also affect miRNA content in EVs. LVs and SVs were isolated from medium of NSC-34 cells, treated or not with the UPS inhibitor MG132 or with the autophagy inhibitor NH4Cl, by differential centrifugation and characterized by Nanosight. MicroRNA libraries were generated using Small RNA-Seq Library Prep Kit (Lexogen) and sequenced on a NextSeq 500/550 (Illumina). Interaction prediction was carried out on TarBase v.8 database. We found a total of 91 Differentially Expressed (DE) (log Fold Change (FC) >1 and <-1) microRNAs in treated-EVs compared to untreated EVs. No DE miRNA were found in NH4Cl-LVs, only 7 miRNA were DE in MG132-LVs and of the 82 miRNAs in MG132-SVs and 66 in NH4Cl-SVs, 43 were in common. Interestingly, the most enriched pathway targeted by commonly DE SVs-miRNAs is the prion disease. In conclusion our observation suggests that, in disease condition, EVs are enriched in both toxic TDP-43 species and potentially harmful miRNA, and thus they may contribute to the propagation of the disease from affected to healthy cells. |
| نوع الوثيقة: |
conference object |
| اللغة: |
English |
| Relation: |
Inflammation and proteinopathy in ALS FTD spectrum disorder; https://hdl.handle.net/2434/950335 |
| الاتاحة: |
https://hdl.handle.net/2434/950335 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.A35C62D1 |
| قاعدة البيانات: |
BASE |