Academic Journal
Immunohistochemistry and polymerase chain reaction on paraffin‐embedded material improve the diagnosis of cutaneous leishmaniasis in the Amazon region
| العنوان: | Immunohistochemistry and polymerase chain reaction on paraffin‐embedded material improve the diagnosis of cutaneous leishmaniasis in the Amazon region |
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| المؤلفون: | Amato, Valdir Sabbaga, Tuon, Felipe Francisco, De Andrade, Jr, Heitor Franco, Bacha, Helio, Pagliari, Carla, Fernandes, Elaine Raniero, Duarte, Maria Irma Seixas, Neto, Vicente Amato, Zampieri, Ricardo Andrade, Floeter‐Winter, Lucile Maria, Celeste, Beatriz J., Oliveira, Juliane, Quiroga, Mariana Martinez, Mascheretti, Melissa, Boulos, Marcos |
| المصدر: | International Journal of Dermatology ; volume 48, issue 10, page 1091-1095 ; ISSN 0011-9059 1365-4632 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2009 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Background Recently, there has been an increase in the incidence of cutaneous leishmaniasis (CL), which represents an important health problem. This increase may be related to the epidemiologic expansion of the infective agent and the increase in tourism in tropical areas. The difficulty in clinical diagnosis, mainly in areas in which CL is not the first consideration of local physicians, has intensified efforts to describe diagnostic tests, which should be specific, sensitive, and practical. Amongst the new tests described are those including nucleic acid amplification (polymerase chain reaction, PCR) and immunohistochemistry (IHC). Methods In this study, we evaluated the sensitivity of a PCR based on small subunit (SSU) ribosomal DNA, in comparison with IHC using Leishmania spp. antibodies, in biopsies embedded in paraffin. Result The results indicated a total sensitivity of 96% (90.9% with PCR and 68.8% with IHC), showing the possibility of using paraffin‐embedded biopsies to diagnose CL. Conclusion We propose the use of the two tests together as a routine protocol for diagnosis. This would require the provision of local medical services to perform molecular biology techniques and adequate Leishmania antibodies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1365-4632.2009.04099.x |
| الاتاحة: | http://dx.doi.org/10.1111/j.1365-4632.2009.04099.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-4632.2009.04099.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-4632.2009.04099.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.A2879322 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1365-4632.2009.04099.x |
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