Academic Journal
SARS-CoV-2 Molecular and Phylogenetic analysis in COVID-19 patients: A preliminary report from Iran
| العنوان: | SARS-CoV-2 Molecular and Phylogenetic analysis in COVID-19 patients: A preliminary report from Iran |
|---|---|
| المؤلفون: | Tabibzadeh, A., Zamani, F., Laali, A., Esghaei, M., Safarnezhad Tameshkel, F., Keyvani, H., Jamshidi Makiani, M., Panahi, M., Motamed, N., Perumal, D., Khoonsari, M., Ajdarkosh, H., Sohrabi, M., Ghanbari, B., Savaj, S., Mosavi-Jarrahi, A., Karbalaie Niya, M.H. |
| سنة النشر: | 2020 |
| المجموعة: | eprints Iran University of Medical Sciences |
| مصطلحات موضوعية: | WC Communicable Diseases |
| الوصف: | Background: The aim of the current study was to investigate and track the SARS-CoV-2 in Iranian Coronavirus Disease 2019 (COVID-19) patients using molecular and phylogenetic methods. Methods: We enrolled seven confirmed cases of COVID-19 patients for the phylogenetic assessment of the SARS-CoV-2 in Iran. The nsp-2, nsp-12, and S genes were amplified using one-step RT-PCR and sequenced using Sanger sequencing method. Popular bioinformatics software were used for sequences alignment and analysis as well as phylogenetic construction. Results: The mean age of the patients in the present study was 60.42 ± 9.94 years and 57.1 (4/7) were male. The results indicated high similarity between Iranian and Chinese strains. We could not find any particular polymorphisms in the assessed regions of the three genes. Phylogenetic trees by neighbor-joining and maximum likelihood method of nsp-2, nsp-12, and S genes showed that there are not any differences between Iranian isolates and those of other countries. Conclusion: As a preliminary phylogenetic study in Iranian SARS-CoV-2 isolates, we found that these isolates are closely related to the Chinese and reference sequences. Also, no sensible differences were observed between Iranian isolates and those of other countries. Further investigations are recommended using more comprehensive methods and larger sample sizes. © 2020 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | Tabibzadeh, A. and Zamani, F. and Laali, A. and Esghaei, M. and Safarnezhad Tameshkel, F. and Keyvani, H. and Jamshidi Makiani, M. and Panahi, M. and Motamed, N. and Perumal, D. and Khoonsari, M. and Ajdarkosh, H. and Sohrabi, M. and Ghanbari, B. and Savaj, S. and Mosavi-Jarrahi, A. and Karbalaie Niya, M.H. (2020) SARS-CoV-2 Molecular and Phylogenetic analysis in COVID-19 patients: A preliminary report from Iran. Infection, Genetics and Evolution, 84. |
| الاتاحة: | http://eprints.iums.ac.ir/33910/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085772742&doi=10.1016%2fj.meegid.2020.104387&partnerID=40&md5=f06e1a8c7e823eb6cb41e008b836917e |
| رقم الانضمام: | edsbas.A17EBB5E |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |