Academic Journal
The Crystal Structure of the Carboxy-Terminal Domain of Human Translation Initiation Factor eIF5
| العنوان: | The Crystal Structure of the Carboxy-Terminal Domain of Human Translation Initiation Factor eIF5 |
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| المؤلفون: | Bieniossek, Christoph, Schütz, Patrick, Bumann, Mario, Limacher, Andreas, Usón, Isabel, Baumann, Ulrich |
| بيانات النشر: | Academic Press |
| سنة النشر: | 2006 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | multi-factor complex, HEAT domain, eIF5, translation initiation |
| الوصف: | The carboxy-terminal domain (CTD) of eukaryotic initiation factor 5 (eIF5) plays a central role in the formation of the multifactor complex (MFC), an important intermediate for the 43 S preinitiation complex assembly. The IF5-CTD interacts directly with the translation initiation factors eIF1, eIF2-β, and eIF3c, thus forming together with eIF2 bound Met-tRNAi Met the MFC. In this work we present the high resolution crystal structure of eIF5-CTD. This domain of the protein is exclusively composed out of alpha-helices and is homologous to the carboxy-terminal domain of eIF2B-ε (eIF2Bε-CTD). The most striking difference in the two structures is an additional carboxy-terminal helix in eIF5. The binding sites of eIF2-β, eIF3 and eIF1 were mapped onto the structure. eIF2-β and eIF3 bind to non-overlapping patches of negative and positive electrostatic potential, respectively. © 2006 Elsevier Ltd. All rights reserved. ; This work has been supported by the Swiss National Science Foundation and the Berner Hochschulstiftung. We gratefully acknowledge the help of Clemens Schulze-Briese at beamline X06SA, SLS, PSI Villigen, Martin Walsh at beamline BM14, ESRF, Grenoble, and Gordon Leonard at ID29, ESRF, Grenoble ; Peer Reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0022-2836 |
| Relation: | http://dx.doi.org/10.1016/j.jmb.2006.05.021; Journal of Molecular Biology 360(2): 457-465 (2006); http://hdl.handle.net/10261/113920 |
| DOI: | 10.1016/j.jmb.2006.05.021 |
| الاتاحة: | http://hdl.handle.net/10261/113920 https://doi.org/10.1016/j.jmb.2006.05.021 |
| Rights: | none |
| رقم الانضمام: | edsbas.A16B028 |
| قاعدة البيانات: | BASE |
| تدمد: | 00222836 |
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| DOI: | 10.1016/j.jmb.2006.05.021 |