Academic Journal
SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids
| العنوان: | SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids |
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| المؤلفون: | Jansen, J, Reimer, KC, Nagai, JS, Varghese, FS, Overheul, GJ, de Beer, M, Roverts, R, Daviran, D, Fermin, LAS, Willemsen, B, Beukenboom, M, Djudjaj, S, von Stillfried, S, van Eijk, LE, Mastik, M, Bulthuis, M, Dunnen, WD, van Goor, H, Hillebrands, J-L, Triana, SH, Alexandrov, T, Timm, M-C, van den Berge, BT, van den Broek, M, Nlandu, Q, Heijnert, J, Bindels, EMJ, Hoogenboezem, RM, Mooren, F, Kuppe, C, Miesen, P, Grünberg, K, Ijzermans, T, Steenbergen, EJ, Czogalla, J, Schreuder, MF, Sommerdijk, N, Akiva, A, Boor, P, Puelles, VG, Floege, J, Huber, TB, van Rij, RP, Costa, IG, Schneider, RK, Smeets, B, Kramann, R |
| المساهمون: | consortium, COVID Moonshot, Zitzmann, N |
| بيانات النشر: | Cell Press |
| سنة النشر: | 2024 |
| المجموعة: | Oxford University Research Archive (ORA) |
| الوصف: | Kidney failure is frequently observed during and after COVID-19, but it remains elusive whether this is a direct effect of the virus. Here, we report that SARS-CoV-2 directly infects kidney cells and is associated with increased tubule-interstitial kidney fibrosis in patient autopsy samples. To study direct effects of the virus on the kidney independent of systemic effects of COVID-19, we infected human-induced pluripotent stem-cell-derived kidney organoids with SARS-CoV-2. Single-cell RNA sequencing indicated injury and dedifferentiation of infected cells with activation of profibrotic signaling pathways. Importantly, SARS-CoV-2 infection also led to increased collagen 1 protein expression in organoids. A SARS-CoV-2 protease inhibitor was able to ameliorate the infection of kidney cells by SARS-CoV-2. Our results suggest that SARS-CoV-2 can directly infect kidney cells and induce cell injury with subsequent fibrosis. These data could explain both acute kidney injury in COVID-19 patients and the development of chronic kidney disease in long COVID. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://ora.ox.ac.uk/objects/uuid:f353299f-4d2d-4fa7-a2ad-c965d2c85615; https://doi.org/10.1016/j.stem.2021.12.010 |
| DOI: | 10.1016/j.stem.2021.12.010 |
| الاتاحة: | https://doi.org/10.1016/j.stem.2021.12.010 https://ora.ox.ac.uk/objects/uuid:f353299f-4d2d-4fa7-a2ad-c965d2c85615 |
| Rights: | info:eu-repo/semantics/openAccess ; CC Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND) |
| رقم الانضمام: | edsbas.A108B14D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.stem.2021.12.010 |
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