Academic Journal
The effects of prostaglandin E-2 treatment on the secretory function of mare corpus luteum depends on the site of application : an in vivo study
| العنوان: | The effects of prostaglandin E-2 treatment on the secretory function of mare corpus luteum depends on the site of application : an in vivo study |
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| المؤلفون: | Piotrowska-Tomala, Katarzyna K., Jonczyk, Agnieszka W., Szostek-Mioduchowska, Anna Z., Zebrowska, Ewelina, Ferreira-Dias, Graça, Skarzynski, Dariusz J. |
| بيانات النشر: | Frontiers Media |
| سنة النشر: | 2022 |
| المجموعة: | Technical University of Lisbon: UTL Repository / Universidade Técnica de Lisboa |
| مصطلحات موضوعية: | Prostaglandin E2, Human chorion gonadotropin, corpus luteum, progesterone, mare |
| الوصف: | Research Areas: Veterinary Sciences ; We examined the effect of prostaglandin (PG) E2 on the secretory function of equine corpus luteum (CL), according to the application site: intra-CL injection vs. an intrauterine (intra-U) administration. Moreover, the effect of intra-CL injection vs. intra-U administration of both luteotropic factors: PGE2 and human chorionic gonadotropin (hCG) as a positive control, on CL function was additionally compared. Mares were assigned to the groups (n = 6 per group): (1) an intra-CL saline injection (control); (2) an intra-CL injection of PGE2 (5 mg/ml); (3) an intra-CL injection of hCG (1,500 IU/ml); (4) an intra-U saline administration (control); (5) an intra-U administration of PGE2 (5 mg/5 ml); (6) an intra-U administration of hCG (1,500 IU/5 ml). Progesterone (P4) and PGE2 concentrations were measured in blood plasma samples collected at −2, −1, and 0 (pre-treatment), and at 1, 2, 3, 4, 6, 8, 10, 12, and 24 h after treatments. Moreover, effects of different doses of PGE2 application on the concentration of total PGF2α (PGF2α and its main metabolite 13,14-dihydro-15-keto-prostaglandin F2α– PGFM) was determined. The time point of PGE2, hCG, or saline administration was defined as hour “0” of the experiment. An intra-CL injection of PGE2 increased P4 and PGE2 concentrations between 3 and 4 h or at 3 and 12 h, respectively (p < 0.05). While intra-U administration of PGE2 elevated P4 concentrations between 8 and 24 h, PGE2 was upregulated at 1 h and between 3 and 4 h (p < 0.05). An intra-CL injection of hCG increased P4 concentrations at 1, 6, and 12 h (p < 0.05), while its intra-U administration enhanced P4 and PGE2 concentrations between 1 and 12 h or at 3 h and between 6 and 10 h, respectively (p < 0.05). An application of PGE2, dependently on the dose, supports equine CL function, regardless of the application site, consequently leading to differences in both P4 and PGE2 concentrations in blood plasma ; info:eu-repo/semantics/publishedVersion |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2297-1769 |
| Relation: | 2011/02/A/NZ5/00338; https://www.frontiersin.org/articles/10.3389/fvets.2021.753796/full; Piotrowska-Tomala KK, Jonczyk AW, Szostek-Mioduchowska AZ, Zebrowska E, Ferreira-Dias G, Skarzynski DJ. 2022. The effects of prostaglandin E-2 treatment on the secretory function of mare corpus luteum depends on the site of application : an in vivo study. Frontiers Veterinary Science, 8:753796. DOI:10.3389/fvets.2021.753796; http://hdl.handle.net/10400.5/24182 |
| DOI: | 10.3389/fvets.2021.753796 |
| الاتاحة: | http://hdl.handle.net/10400.5/24182 https://doi.org/10.3389/fvets.2021.753796 |
| Rights: | openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.A0CED757 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 22971769 |
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| DOI: | 10.3389/fvets.2021.753796 |