Academic Journal
Insertion-sequence-mediated mutations both promote and constrain evolvability during a long-term experiment with bacteria
| العنوان: | Insertion-sequence-mediated mutations both promote and constrain evolvability during a long-term experiment with bacteria |
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| المؤلفون: | Consuegra, Jessika, Gaffé, Joël, Lenski, Richard E., Hindré, Thomas, Barrick, Jeffrey E., Tenaillon, Olivier, Schneider, Dominique |
| المساهمون: | European Commission, Agence Nationale de la Recherche, Fondation pour la Recherche Médicale |
| المصدر: | Nature Communications ; volume 12, issue 1 ; ISSN 2041-1723 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2021 |
| الوصف: | Insertion sequences (IS) are ubiquitous bacterial mobile genetic elements, and the mutations they cause can be deleterious, neutral, or beneficial. The long-term dynamics of IS elements and their effects on bacteria are poorly understood, including whether they are primarily genomic parasites or important drivers of adaptation by natural selection. Here, we investigate the dynamics of IS elements and their contribution to genomic evolution and fitness during a long-term experiment with Escherichia coli . IS elements account for ~35% of the mutations that reached high frequency through 50,000 generations in those populations that retained the ancestral point-mutation rate. In mutator populations, IS-mediated mutations are only half as frequent in absolute numbers. In one population, an exceptionally high ~8-fold increase in IS 150 copy number is associated with the beneficial effects of early insertion mutations; however, this expansion later slowed down owing to reduced IS 150 activity. This population also achieves the lowest fitness, suggesting that some avenues for further adaptation are precluded by the IS 150 -mediated mutations. More generally, across all populations, we find that higher IS activity becomes detrimental to adaptation over evolutionary time. Therefore, IS-mediated mutations can both promote and constrain evolvability. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41467-021-21210-7 |
| الاتاحة: | http://dx.doi.org/10.1038/s41467-021-21210-7 https://www.nature.com/articles/s41467-021-21210-7.pdf https://www.nature.com/articles/s41467-021-21210-7 |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.A0A1297D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-021-21210-7 |
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