Academic Journal
Consequences of interleukin 1β‐triggered chronic inflammation in the mouse prostate gland: Altered architecture associated with prolonged CD4 + infiltration mimics human proliferative inflammatory atrophy
| العنوان: | Consequences of interleukin 1β‐triggered chronic inflammation in the mouse prostate gland: Altered architecture associated with prolonged CD4 + infiltration mimics human proliferative inflammatory atrophy |
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| المؤلفون: | Ashok, Arya, Keener, Rebecca, Rubenstein, Michael, Stookey, Stephanie, Bajpai, Sagar, Hicks, Jessica, Alme, Angela K., Drake, Charles G., Zheng, Qizhi, Trabzonlu, Levent, Yegnasubramanian, Srinivasan, De Marzo, Angelo M., Bieberich, Charles J. |
| المساهمون: | Foundation for the National Institutes of Health, Prostate Cancer Foundation, Congressionally Directed Medical Research Programs |
| المصدر: | The Prostate ; volume 79, issue 7, page 732-745 ; ISSN 0270-4137 1097-0045 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2019 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Background Elevated expression of the proinflammatory cytokine interleukin 1β (IL‐1β) has been observed in expressed prostatic secretions of patients with chronic prostatitis/chronic pelvic pain syndrome, and genetic polymorphisms associated with the IL1B gene are linked to increased risk for aggressive prostate cancer. Methods To study the role of IL‐1β expression in prostate inflammation, we examined IL1B expression in human prostatic proliferative inflammatory atrophy (PIA) lesions and developed a tetracycline‐regulated human IL1B transgene in the mouse prostate. Results Here, we demonstrate that IL1B expression is a common finding in human PIA lesions, which harbored focal IL1B expression in epithelial and stromal compartments. Human IL1B expression in the mouse prostate elicited acute and chronic inflammation. Penetrance and expressivity were variable and tunable by altering transgene dosage and the presence of an exogenous inducible marker antigen (green fluorescent protein). Inflammation was characterized by infiltration of CD4 + T cells, demonstrating an adaptive immune response. Chronic inflammation persisted after doxycycline (Dox) withdrawal. Reactive epithelia increased expression of downstream cytokines, and altered glandular architecture was observed upon sustained induction of IL1B . Immunohistochemical analyses revealed a higher proliferative index and decreased Nkx3.1 expression in inflamed mouse prostates. Conclusions These data implicate IL‐1β in human prostate pathology and this model provides a versatile platform to interrogate molecular mechanisms of inflammation‐associated prostate pathologies associated with episodic or sustained IL‐1β expression. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/pros.23784 |
| الاتاحة: | http://dx.doi.org/10.1002/pros.23784 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fpros.23784 https://onlinelibrary.wiley.com/doi/pdf/10.1002/pros.23784 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/pros.23784 https://onlinelibrary.wiley.com/doi/am-pdf/10.1002/pros.23784 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#am ; http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.A032AA51 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/pros.23784 |
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