Academic Journal
Heterogeneity of proliferative markers in pancreatic β-cells of patients with severe hypoglycemia following Roux-en-Y gastric bypass.
| العنوان: | Heterogeneity of proliferative markers in pancreatic β-cells of patients with severe hypoglycemia following Roux-en-Y gastric bypass. |
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| المؤلفون: | Patti, M.E., Goldfine, A.B., Hu, J., Hoem, D., Molven, A., Goldsmith, J., Schwesinger, W.H., La Rosa, S., Folli, F., Kulkarni, R.N. |
| المصدر: | Acta diabetologica, vol. 54, no. 8, pp. 737-747 |
| سنة النشر: | 2017 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: | Adult, Aged, Blood Glucose/metabolism, Cell Proliferation, Female, Gastric Bypass/adverse effects, Gastric Inhibitory Polypeptide/metabolism, Humans, Hypoglycemia/etiology, Hypoglycemia/metabolism, Hypoglycemia/physiopathology, Incretins/metabolism, Insulin/secretion, Insulin-Secreting Cells/cytology, Insulin-Secreting Cells/metabolism, Male, Middle Aged, Pancreas/metabolism, Gastro-entero pancreatic factors, Human, Hypoglycemia, Islet |
| الوصف: | Severe postprandial hypoglycemia with neuroglycopenia is an increasingly recognized, debilitating complication of Roux-en-Y gastric bypass (RYGB) surgery. Increased secretion of insulin and incretin hormones is implicated in its pathogenesis. Histopathologic examination of pancreas has demonstrated increased islet size and/or nuclear diameter in post-RYGB patients who underwent pancreatectomy for severe refractory hypoglycemia with neuroglycopenia (RYGB + NG). We aimed to determine whether β-cell proliferation or apoptosis is altered in RYGB + NG. We performed an observational study to analyze markers of proliferation, apoptosis, cell cycle, and transcription factor expression in pancreatic tissue from affected RYGB + NG patients (n = 12), normoglycemic patients undergoing pancreatic surgery for benign lesions (controls, n = 6), and individuals with hypoglycemia due to insulinoma (n = 52). Proliferative cell nuclear antigen (PCNA) expression was increased in insulin-positive cells in RYGB + NG patients (4.5-fold increase, p < 0.001 vs. controls) and correlated with β-cell mass. Ki-67 immunoreactivity was low in both RYGB + NG and controls, but did not differ between groups. Phospho-histone H3 levels did not differ between RYGB + NG and controls. PCNA and Ki-67 were both significantly lower in both controls and RYGB + NG than insulinomas. Markers of apoptosis and cell cycle (M30, p27, and p21) did not differ between groups. PDX1 and menin exhibited similar expression patterns, while FOXO1 appeared to be more cytosolic in RYGB + NG. Markers of proliferation are heterogeneous in patients with severe post-RYGB hypoglycemia. Increased β-cell proliferation in some individuals may contribute to increased β-cell mass observed in severely affected patients. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/28512677; info:eu-repo/semantics/altIdentifier/eissn/1432-5233; info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_B43F8666B75B6; https://serval.unil.ch/notice/serval:BIB_B43F8666B75B; https://serval.unil.ch/resource/serval:BIB_B43F8666B75B.P001/REF.pdf |
| DOI: | 10.1007/s00592-017-1001-2 |
| الاتاحة: | https://serval.unil.ch/notice/serval:BIB_B43F8666B75B https://doi.org/10.1007/s00592-017-1001-2 https://serval.unil.ch/resource/serval:BIB_B43F8666B75B.P001/REF.pdf http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_B43F8666B75B6 |
| Rights: | info:eu-repo/semantics/openAccess ; Copying allowed only for non-profit organizations ; https://serval.unil.ch/disclaimer |
| رقم الانضمام: | edsbas.A02572EA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00592-017-1001-2 |
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