Academic Journal
Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary culture of oral keratinocytes
| العنوان: | Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary culture of oral keratinocytes |
|---|---|
| المؤلفون: | Flavia Maziero Andreghetto, Maria Fatima Guarizo Klingbeil, Renata Machado Soares, Roberta Sitnik, Décio dos Santos Pinto Junior, Monica Beatriz Mathor, Fabio Daumas Nunes, Patricia Severino |
| المصدر: | Einstein (São Paulo), Vol 9, Iss 4, Pp 442-448 (2011) |
| بيانات النشر: | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| سنة النشر: | 2011 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Gene expression, Keratinocytes, Carcinoma, squamous cell, Head and neck neoplasms, Medicine |
| الوصف: | Objective: Functional in vitro studies are fundamental to understand the role of microRNAs, small non coding RNA molecules that function as post-transcriptional regulators, in cancer. The objective of this study was to determine the applicability of head and neck squamous cell carcinoma cell lines and human oral keratinocytes as models for functional studies on microRNAs previously identified as deregulated in head and neck squamous cell carcinomas. Methods: The expression level of four microRNAs was assessed in cell lines and in primary cultures of oral keratinocytes using specific real-time polymerase chain reactions. The identity of oral squamous cell carcinoma cell lines was confirmed by means of STR (short tandem repeats) profiling. The possible impact of feeder-layer gene expression in global microRNA expression results from keratinocyte primary culture was also evaluated. Results: Significant differences in microRNA gene expression were observed among squamous cell carcinoma cell lines, particularly among cells lines from distinct subsites, as well as between primary culture of human keratinocytes and immortalized keratinocyte cell lines. Conclusions: Primary cultures of human keratinocytes and diverse tumor cell lines are relatively easy to obtain. However, each cell model possesses a characteristic phenotype; whereas one may be useful for a specific study, it may be inappropriate for another. Therefore, it is imperative that suitable cell lines are cautiously selected for functional studies in cancer. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English Portuguese |
| تدمد: | 2317-6385 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082011000400442&tlng=en; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082011000400442&tlng=pt; https://doaj.org/toc/2317-6385; https://doaj.org/article/08eae87c6b6d463baf4e6a73fa55a4f4 |
| DOI: | 10.1590/s1679-45082011ao2149 |
| الاتاحة: | https://doi.org/10.1590/s1679-45082011ao2149 https://doaj.org/article/08eae87c6b6d463baf4e6a73fa55a4f4 |
| رقم الانضمام: | edsbas.A01D57D2 |
| قاعدة البيانات: | BASE |
| تدمد: | 23176385 |
|---|---|
| DOI: | 10.1590/s1679-45082011ao2149 |