Academic Journal
Control of the heparosan N-deacetylation leads to an improved bioengineered heparin
| العنوان: | Control of the heparosan N-deacetylation leads to an improved bioengineered heparin |
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| المؤلفون: | Wang, Zhenyu, Yang, Bo, Zhang, Zhenqing, Ly, Mellisa, Takieddin, Majde, Mousa, Shaker, Liu, Jian, Dordick, Jonathan S., Linhardt, Robert J. |
| المصدر: | Applied Microbiology and Biotechnology, 91(1) |
| سنة النشر: | 2011 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | Boyau, Artiodactyla, Désacétylation, Héparine, Heparosan, Vertebrata, Ungulata, Pig, Porcine intestine, Intestine, Deacetylation, Heparina, Mammalia, Desacetilación, Porc, Cerdo, Tripa, Heparin |
| الوصف: | The production of the anticoagulant drug heparin from non-animal sources has a number of advantages over the current commercial production of heparin. These advantages include better source material availability, improved quality control, and reduced concerns about animal virus or prion impurities. A bioengineered heparin would have to be chemically and biologically equivalent to be substituted for animal-sourced heparin as a pharmaceutical. In an effort to produce bioengineered heparin that more closely resembles pharmaceutical heparin, we have investigated a key step in the process involving the N-deacetylation of heparosan. The extent of N-deacetylation directly affects the N-acetyl/N-sulfo ratio in bioengineered heparin and also impacts its molecular weight. Previous studies have demonstrated that the presence and quantity of N-acetylglucosamine in the nascent glycosaminoglycan chain, serving as the substrate for the subsequent enzymatic modifications (C5 epimerization and O-sulfonation), can impact the action of these enzymes and, thus, the content and distribution of iduronic acid and O-sulfo groups. In this study, we control the N-deacetylation of heparosan to produce a bioengineered heparin with an N-acetyl/N-sulfo ratio and molecular weight that is similar to animal-sourced pharmaceutical heparin. The structural composition and anticoagulant activity of the resultant bioengineered heparin was extensively characterized and compared to pharmaceutical heparin obtained from porcine intestinal mucosa. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://doi.org/10.17615/m6jc-hg77; https://cdr.lib.unc.edu/downloads/jq085t51d?file=thumbnail; https://cdr.lib.unc.edu/downloads/jq085t51d |
| DOI: | 10.17615/m6jc-hg77 |
| الاتاحة: | https://doi.org/10.17615/m6jc-hg77 https://cdr.lib.unc.edu/downloads/jq085t51d?file=thumbnail https://cdr.lib.unc.edu/downloads/jq085t51d |
| Rights: | http://rightsstatements.org/vocab/InC/1.0/ |
| رقم الانضمام: | edsbas.9FDF20D9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/m6jc-hg77 |
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