Academic Journal
Fluorescent fatty acid conjugates for live cell imaging of peroxisomes.
| العنوان: | Fluorescent fatty acid conjugates for live cell imaging of peroxisomes. |
|---|---|
| المؤلفون: | Korotkova, D., Borisyuk, A., Guihur, A., Bardyn, M., Kuttler, F., Reymond, L., Schuhmacher, M., Amen, T. |
| المصدر: | Nature communications, vol. 15, no. 1, pp. 4314 |
| سنة النشر: | 2024 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: | Peroxisomes/metabolism, Humans, Fatty Acids/metabolism, Fluorescent Dyes/chemistry, Boron Compounds/chemistry, Peroxisomal Disorders/metabolism, Animals |
| الوصف: | Peroxisomes are eukaryotic organelles that are essential for multiple metabolic pathways, including fatty acid oxidation, degradation of amino acids, and biosynthesis of ether lipids. Consequently, peroxisome dysfunction leads to pediatric-onset neurodegenerative conditions, including Peroxisome Biogenesis Disorders (PBD). Due to the dynamic, tissue-specific, and context-dependent nature of their biogenesis and function, live cell imaging of peroxisomes is essential for studying peroxisome regulation, as well as for the diagnosis of PBD-linked abnormalities. However, the peroxisomal imaging toolkit is lacking in many respects, with no reporters for substrate import, nor cell-permeable probes that could stain dysfunctional peroxisomes. Here we report that the BODIPY-C12 fluorescent fatty acid probe stains functional and dysfunctional peroxisomes in live mammalian cells. We then go on to improve BODIPY-C12, generating peroxisome-specific reagents, PeroxiSPY650 and PeroxiSPY555. These probes combine high peroxisome specificity, bright fluorescence in the red and far-red spectrum, and fast non-cytotoxic staining, making them ideal tools for live cell, whole organism, or tissue imaging of peroxisomes. Finally, we demonstrate that PeroxiSPY enables diagnosis of peroxisome abnormalities in the PBD CRISPR/Cas9 cell models and patient-derived cell lines. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38773129; info:eu-repo/semantics/altIdentifier/eissn/2041-1723; info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_65E6AB1AE38B9; https://serval.unil.ch/notice/serval:BIB_65E6AB1AE38B; https://serval.unil.ch/resource/serval:BIB_65E6AB1AE38B.P001/REF.pdf |
| DOI: | 10.1038/s41467-024-48679-2 |
| الاتاحة: | https://serval.unil.ch/notice/serval:BIB_65E6AB1AE38B https://doi.org/10.1038/s41467-024-48679-2 https://serval.unil.ch/resource/serval:BIB_65E6AB1AE38B.P001/REF.pdf http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_65E6AB1AE38B9 |
| Rights: | info:eu-repo/semantics/openAccess ; CC BY 4.0 ; https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.9FACBD67 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-024-48679-2 |
|---|