Academic Journal
Generation of integration-free induced pluripotent stem cells from a patient with spina bifida
| العنوان: | Generation of integration-free induced pluripotent stem cells from a patient with spina bifida |
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| المؤلفون: | Hongran Wang, Shuying Zhao, Richard H. Finnell, Timothy George, Austin J. Cooney |
| المصدر: | Stem Cell Research, Vol 31, Iss , Pp 27-30 (2018) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2018 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | A skin biopsy was obtained from a 14-year-old female patient with a history of Myelomeningocele. Dermal fibroblasts were isolated and reprogrammed with Sendai virus (SeV) vectors encoding OCT3/4, SOX2, KLF4, and c-MYC. The generated induced Pluripotent Stem Cell (iPSC) clones NTDi4_09A were free of genomically integrated reprogramming genes, had a stable normal karyotype and expressed pluripotency markers. The iPSCs formed teratomas in mice, which were differentiated towards derivatives of the three germ layers in vivo. This iPSC line offers a useful resource to study a genetic profile of a patient with spina bifida. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1873-5061 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1873506118301259; https://doaj.org/toc/1873-5061; https://doaj.org/article/f28e840d097141bc8872bc3f217413d1 |
| DOI: | 10.1016/j.scr.2018.05.007 |
| الاتاحة: | https://doi.org/10.1016/j.scr.2018.05.007 https://doaj.org/article/f28e840d097141bc8872bc3f217413d1 |
| رقم الانضمام: | edsbas.9FA606D3 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 18735061 |
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| DOI: | 10.1016/j.scr.2018.05.007 |