التفاصيل البيبلوغرافية
| العنوان: |
A two-step scaffolding model for mitotic chromosome assembly |
| المؤلفون: |
Maeshima, Kazuhiro, Laemmli, Ulrich Karl |
| المصدر: |
ISSN: 1534-5807 ; Developmental cell, vol. 4, no. 4 (2003) p. 467-480. |
| سنة النشر: |
2003 |
| المجموعة: |
Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: |
info:eu-repo/classification/ddc/540, info:eu-repo/classification/ddc/570, Adenosine Diphosphate / genetics, Adenosine Diphosphate / metabolism, Adenosine Triphosphatases / genetics, Adenosine Triphosphatases / metabolism, Adenosine Triphosphate / genetics, Adenosine Triphosphate / metabolism, Antigens, Neoplasm, Cell Cycle Proteins / genetics, Cell Cycle Proteins / metabolism, Cell Nucleus / enzymology, Cell Nucleus / ultrastructure, Chromatids / genetics, Chromatids / ultrastructure, Chromosomes / enzymology, Chromosomes / ultrastructure, DNA Topoisomerases, Type II / genetics, Type II / metabolism, DNA-Binding Proteins / genetics, DNA-Binding Proteins / metabolism, Eukaryotic Cells / enzymology, Eukaryotic Cells / ultrastructure, Fluorescent Antibody Technique, HeLa Cells, Humans, Microtomy, Mitosis / genetics |
| الوصف: |
Topoisomerase IIalpha (topoIIalpha) and 13S condensin are both required for mitotic chromosome assembly. Here we show that they constitute the two main components of the chromosomal scaffold on histone-depleted chromosomes. The structural stability and chromosomal shape of the scaffolding toward harsh extraction procedures are shown to be mediated by ATP or its nonhydrolyzable analogs, but not ADP. TopoIIalpha and 13S condensin components immunolocalize to a radially restricted, longitudinal scaffolding in native-like chromosomes. Double staining for topoIIalpha and condensin generates a barber pole appearance of the scaffolding, where topoIIalpha- and condensin-enriched "beads" alternate; this structure appears to be generated by two juxtaposed, or coiled, chains. Cell cycle studies establish that 13S condensin appears not to be involved in the assembly of prophase chromatids; they lack this complex but contain a topoIIalpha-defined (-mediated?) scaffolding. Condensin associates only during the pro- to metaphase transition. This two-step assembly process is proposed to generate the barber pole appearance of the native-like scaffolding. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/12689587; https://archive-ouverte.unige.ch/unige:167473; unige:167473 |
| الاتاحة: |
https://archive-ouverte.unige.ch/unige:167473 |
| Rights: |
info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: |
edsbas.9F68721 |
| قاعدة البيانات: |
BASE |