Academic Journal
Immunoinformatics and molecular dynamics approaches: Next generation vaccine design against West Nile virus
| العنوان: | Immunoinformatics and molecular dynamics approaches: Next generation vaccine design against West Nile virus |
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| المؤلفون: | Khan, Md Tahsin, Islam, Rahatul, Jerin, Tarhima Jahan, Mahmud, Araf, Khatun, Sahara, Kobir, Ahasanul, Islam, Md Nahidul, Akter, Arzuba, Mondal, Shakhinur Islam |
| المساهمون: | Tang, Jianming |
| المصدر: | PLOS ONE ; volume 16, issue 6, page e0253393 ; ISSN 1932-6203 |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2021 |
| المجموعة: | PLOS Publications (via CrossRef) |
| الوصف: | West Nile Virus (WNV) is a life threatening flavivirus that causes significant morbidity and mortality worldwide. No preventive therapeutics including vaccines against WNV are available for human use. In this study, immunoinformatics approach was performed to design a multi epitope-based subunit vaccine against this deadly pathogen. Human (HLA) and Mice (H-2) allele specific potential T-cell and B-cell epitopes were shortlisted through a stringent procedure. Molecular docking showed selected epitopes that have stronger binding affinity with human TLR-4. Molecular dynamics simulation confirmed the stable nature of the docked complex. Furthermore, in silico cloning analysis ensures efficient expression of desired gene in the microbial system. Interestingly, previous studies showed that two of our selected epitopes have strong immune response against WNV. Therefore, selected epitopes could be strong vaccine candidates to prevent WNV infections in human. However, further in vitro and in vivo investigations could be strengthening the validation of the vaccine candidate against WNV. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1371/journal.pone.0253393 |
| الاتاحة: | http://dx.doi.org/10.1371/journal.pone.0253393 https://dx.plos.org/10.1371/journal.pone.0253393 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.9F08C21B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0253393 |
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