Academic Journal
How Low Can It Go? ATR-FTIR Characterization of Compounds Isolated from Ginger at the Nanogram Level
| العنوان: | How Low Can It Go? ATR-FTIR Characterization of Compounds Isolated from Ginger at the Nanogram Level |
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| المؤلفون: | Joel B. Johnson, Ryan J. Batley, Janice S. Mani, Mani Naiker |
| المصدر: | Engineering Proceedings, Vol 56, Iss 1, p 80 (2023) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | detection limit, structural elucidation, natural products, Engineering machinery, tools, and implements, TA213-215 |
| الوصف: | This proof-of-concept study demonstrated the potential of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy for the structural characterization of natural products when only very small quantities of the target compound are available. Four known compounds (6-gingerol, 6-shogaol, 8-gingerol and 10-gingerol) were isolated from ginger ( Zingiber officinale ) rhizome using semi-preparative high-performance liquid chromatography (HPLC). A portion of each fraction was evaporated on the ATR plate and spectra collected using a standard FTIR instrument. The minimum amount required to detect some spectral features appeared to be around 50 ng for the gingerols, and around 25 ng for 6-shogaol. Various peaks are assigned and interpreted to demonstrate the range of structural information that can be obtained. Evaporation-based ATR-FTIR spectroscopy could be an inexpensive and rapid method to aid structural elucidation of natural compounds, even when collected from a single semi-preparative HPLC run. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2673-4591 |
| Relation: | https://www.mdpi.com/2673-4591/56/1/80; https://doaj.org/toc/2673-4591; https://doaj.org/article/2ea905bcb55f4a82a04fc20fbc0a9668 |
| DOI: | 10.3390/ASEC2023-15407 |
| الاتاحة: | https://doi.org/10.3390/ASEC2023-15407 https://doaj.org/article/2ea905bcb55f4a82a04fc20fbc0a9668 |
| رقم الانضمام: | edsbas.9E49ECB |
| قاعدة البيانات: | BASE |
| تدمد: | 26734591 |
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| DOI: | 10.3390/ASEC2023-15407 |