Academic Journal

Spectrally Resolved Fundus Autofluorescence in Healthy Eyes: Repeatability and Topographical Analysis of the Green-Emitting Fluorophores

التفاصيل البيبلوغرافية
العنوان: Spectrally Resolved Fundus Autofluorescence in Healthy Eyes: Repeatability and Topographical Analysis of the Green-Emitting Fluorophores
المؤلفون: Enrico Borrelli, Marco Battista, Biancamaria Zuccaro, Riccardo Sacconi, Maria Brambati, Lea Querques, Francesco Prascina, SriniVas R. Sadda, Francesco Bandello, Giuseppe Querques
المصدر: Journal of Clinical Medicine; Volume 9; Issue 8; Pages: 2388
بيانات النشر: Multidisciplinary Digital Publishing Institute
سنة النشر: 2020
المجموعة: MDPI Open Access Publishing
مصطلحات موضوعية: autofluorescence, green-emitting fluorophores, metabolic study
الوصف: The aim of this study was to report normal measurements of green-emitting fluorophores in the macula of healthy young individuals and to assess the repeatability of these quantitative metrics. To do so, healthy young volunteers were imaged twice (7 ± 3 days apart) using a confocal blue-light fundus autofluorescence (FAF) device with a shorter excitation wavelength (peak at 450 nm) and the capability for separately detecting the red and green components of the emission spectrum. The main outcome measure was the percentage of area occupied by green-emitting fluorophores in the macula. In addition, this measure was performed in separate regions providing a topographical assessment in the foveal, parafoveal and perifoveal regions. Furthermore, the level of agreement between repeated measurements was evaluated. Thirty eyes from 30 healthy volunteers were included in this analysis. Mean age was 26.2 ± 2.8 years (median: 25.0 years; range: 23.0–32.0 years). Median (interquartile range—IQR) area occupied by green-emitting fluorophores was 3.6% (1.9–4.7%) in the macular region. In the topographical analysis, this percentage was higher in the foveal area (median = 33.3%, IQR = 21.9–41.2%), as compared with both the parafoveal (median = 5.3%; IQR = 2.4–8.1%; p < 0.0001) and perifoveal (median = 0.5%, IQR = 0.2–0.8%; p < 0.0001) regions. The coefficient of variation (CV; ranging from 1.1% to 1.7% in the analyzed regions) and the intraclass correlation coefficient (ICC; ranging from 0.93 to 0.97) indicated high levels of repeatability. In conclusion, the assessment of green-emitting fluorophores is repeatable. The distribution of these fluorophores is highest in the foveal region. Assuming that flavin adenine dinucleotide (FAD) emits in the green autofluorescence spectrum, this variability could be secondary to an increased quantity of mitochondria in the foveal region.
نوع الوثيقة: text
وصف الملف: application/pdf
اللغة: English
Relation: Ophthalmology; https://dx.doi.org/10.3390/jcm9082388
DOI: 10.3390/jcm9082388
الاتاحة: https://doi.org/10.3390/jcm9082388
Rights: https://creativecommons.org/licenses/by/4.0/
رقم الانضمام: edsbas.9DB32B4
قاعدة البيانات: BASE