Academic Journal
Site-selected in situ polymerization for living cell surface engineering
| العنوان: | Site-selected in situ polymerization for living cell surface engineering |
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| المؤلفون: | Zhong, Yihong, Xu, Lijia, Yang, Chen, Xu, Le, Wang, Guyu, Guo, Yuna, Cheng, Songtao, Tian, Xiao, Wang, Changjiang, Xie, Ran, Wang, Xiaojian, Ding, Lin, Ju, Huangxian |
| المساهمون: | National Natural Science Foundation of China, NJU | State Key Laboratory of Analytical Chemistry for Life Science, Fundamental Research Funds for the Central Universities |
| المصدر: | Nature Communications ; volume 14, issue 1 ; ISSN 2041-1723 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| الوصف: | The construction of polymer-based mimicry on cell surface to manipulate cell behaviors and functions offers promising prospects in the field of biotechnology and cell therapy. However, precise control of polymer grafting sites is essential to successful implementation of biomimicry and functional modulation, which has been overlooked by most current research. Herein, we report a biological site-selected, in situ controlled radical polymerization platform for living cell surface engineering. The method utilizes metabolic labeling techniques to confine the growth sites of polymers and designs a Fenton-RAFT polymerization technique with cytocompatibility. Polymers grown at different sites (glycans, proteins, lipids) have different membrane retention time and exhibit differential effects on the recognition behaviors of cellular glycans. Of particular importance is the achievement of in situ copolymerization of glycomonomers on the outermost natural glycan sites of cell membrane, building a biomimetic glycocalyx with distinct recognition properties. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41467-023-43161-x |
| الاتاحة: | http://dx.doi.org/10.1038/s41467-023-43161-x https://www.nature.com/articles/s41467-023-43161-x.pdf https://www.nature.com/articles/s41467-023-43161-x |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.9D99E195 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-023-43161-x |
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