Academic Journal
Genome-wide discovery of circulating cell-free DNA methylation biomarkers for colorectal cancer detection
| العنوان: | Genome-wide discovery of circulating cell-free DNA methylation biomarkers for colorectal cancer detection |
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| المؤلفون: | Fang, Qingxiao, Yuan, Ziming, Hu, Hanqing, Zhang, Weiyuan, Wang, Guiyu, Wang, Xishan |
| المساهمون: | National Natural Science Foundation of China, Applied Technology Research and Development Plan of Heilongjiang Province |
| المصدر: | Clinical Epigenetics ; volume 15, issue 1 ; ISSN 1868-7083 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2023 |
| الوصف: | Background Colorectal polyp is known a precursor of colorectal cancer (CRC) that holds an increased risk for progression to CRC. Circulating cell-free DNA (cfDNA) methylation has shown favorable performance in the detection and monitoring the malignant progression in a variety of cancers. Results To discover cfDNA methylation markers for the diagnosis of CRC, we first performed a genome-wide analysis between eight CRC and eight polyp tissues using the Infinium HumanMethylationEPIC BeadChip. We identified 7008 DMCs, and after filtering, we validated 39 DMCs by MethylTarget sequencing in 62 CRC and 56 polyp tissues. A panel of four CpGs (cg04486886, cg06712559, cg13539460, and cg27541454) was selected as the methylation marker in tissue by LASSO and random forest models. A diagnosis prediction model was built based on the four CpGs, and the methylation diagnosis score (md-score) can effectively discriminate tissues with CRC from polyp patients (AUROC > 0.9). Finally, the cg27541454 was confirmed hypermethylated in CRC (AUC = 0.85) in the plasma validation cohort. Conclusions Our findings suggest that the md-score could robustly detect CRC from polyp tissues, and cg27541454 may be a promising candidate noninvasive biomarker for CRC early diagnosis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s13148-023-01518-5 |
| DOI: | 10.1186/s13148-023-01518-5.pdf |
| DOI: | 10.1186/s13148-023-01518-5/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s13148-023-01518-5 https://link.springer.com/content/pdf/10.1186/s13148-023-01518-5.pdf https://link.springer.com/article/10.1186/s13148-023-01518-5/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.9D5E3E98 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s13148-023-01518-5 |
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