Academic Journal
Brief Report: A Bioassay to Identify Primary Human Prostate Cancer Repopulating Cells
| العنوان: | Brief Report: A Bioassay to Identify Primary Human Prostate Cancer Repopulating Cells |
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| المؤلفون: | Toivanen, Roxanne, Berman, David M., Wang, Hong, Pedersen, John, Frydenberg, Mark, Meeker, Alan K., Ellem, Stuart J., Risbridger, Gail P., Taylor, Renea A. |
| المساهمون: | National Health and Medical Research Council of Australia, Prostate Cancer Foundation of Australia, Victorian Prostate Cancer Research Consortium, US Department of Defense |
| المصدر: | Stem Cells ; volume 29, issue 8, page 1310-1314 ; ISSN 1066-5099 1549-4918 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2011 |
| الوصف: | Cancer cells are heterogeneous in both their phenotypes and ability to promote tumor growth and spread. Xenografting is used to identify the most highly capable cells of regenerating tumors, referred to as cancer repopulating cells. Because prostate cancers (PCa's) rarely grow as xenografts, indentifying PCa repopulating cells has not been possible. Here, we report improved methods to xenograft localized primary PCa tissues using chimeric grafts with neonatal mouse mesenchyme. Xenograft survival of tumor tissue was significantly increased by neonatal mesenchyme (six of six patients, 66% of grafts, versus four of six patients, 41% of grafts) and doubled the proliferation index of xenografted cancer cells. When applied to isolated PCa cells, neonatal mesenchyme effectively reconstituted PCa's and increased xenograft survival (four of nine patients; 32% of grafts with mesenchyme and 0% without), and supported active cancer cell proliferation. Using this assay, we showed that unfractionated α2β1integrinhi and α2β1integrinlo cells from primary localized PCa's demonstrated tumor formation at comparable rates, similar to previous reports using metastatic specimens. Thus, this new protocol efficiently established tumors and enabled proliferative expansion of both intact tumor tissue and fractionated cancer cells, providing a bioassay to identify and therapeutically target PCa repopulating cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/stem.668 |
| الاتاحة: | https://doi.org/10.1002/stem.668 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fstem.668 https://onlinelibrary.wiley.com/doi/pdf/10.1002/stem.668 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/stem.668 https://academic.oup.com/stmcls/article-pdf/29/8/1310/41967807/stmcls_29_8_1310.pdf |
| Rights: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| رقم الانضمام: | edsbas.9D33AB1E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/stem.668 |
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