Academic Journal
The Bacillus phage SPβ and its relatives
| العنوان: | The Bacillus phage SPβ and its relatives |
|---|---|
| المؤلفون: | Kohm, Katharina, Floccari, Valentina Andrea, Lutz, Veronika T., Nordmann, Birthe, Mittelstädt, Carolin, Poehlein, Anja, Dragoš, Anna, Commichau, Fabian M., Hertel, Robert |
| المصدر: | Environmental microbiology, vol. 24, no. 4, pp. 2098-2118, 2022. ; ISSN: 1462-2912 |
| بيانات النشر: | Society for Applied Microbiology and John Wiley & Sons |
| سنة النشر: | 2022 |
| المجموعة: | University of Ljubljana: Repository (RUJ) / Repozitorij Univerze v Ljubljani |
| مصطلحات موضوعية: | Bacillus subtilis, profagi, divji tip sevov, lizogenija, genomika, info:eu-repo/classification/udc/579.852.11:578.347:575.111 |
| الوصف: | The Bacillus phage SPβ has been known for about 50 years, but only a few strains are available. We isolated four new wild-type strains of the SPbeta species. Phage vB_BsuS-Goe14 introduces its prophage into the spoVK locus, previously not observed to be used by SPβ-like phages. Sequence data revealed the genome replication strategy and the genome packaging mode of SPβ-like phages. We extracted 55 SPβ-like prophages from public Bacillus genomes, thereby discovering three more integration loci and one additional type of integrase. The identified prophages resemble four new species clusters and three species orphans in the genus Spbetavirus. The determined core proteome of all SPβ-like prophages consists of 38 proteins. The integration cassette proved to be not conserved, even though, present in all strains. It consists of distinct integrases. Analysis of SPβ transcriptomes revealed three conserved genes, yopQ, yopR, and yokI, to be transcribed from a dormant prophage. While yopQ and yokI could be deleted from the prophage without activating the prophage, damaging of yopR led to a clear-plaque phenotype. Under the applied laboratory conditions, the yokI mutant showed an elevated virion release implying the YokI protein being a component of the arbitrium system. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf; text/url |
| اللغة: | English |
| Relation: | info:eu-repo/grantAgreement/other/Volkswagen Foundation/94045; info:eu-repo/grantAgreement/other/Max Buchner Reserch Foundation/3799; info:eu-repo/grantAgreement/ARRS//N1-0177; https://repozitorij.uni-lj.si/IzpisGradiva.php?id=138602; https://repozitorij.uni-lj.si/Dokument.php?id=158974&dn=; https://repozitorij.uni-lj.si/Dokument.php?id=158973&dn=; https://plus.cobiss.net/cobiss/si/sl/bib/101899011; http://hdl.handle.net/20.500.12556/RUL-138602 |
| الاتاحة: | https://repozitorij.uni-lj.si/IzpisGradiva.php?id=138602 https://repozitorij.uni-lj.si/Dokument.php?id=158974&dn= https://repozitorij.uni-lj.si/Dokument.php?id=158973&dn= https://plus.cobiss.net/cobiss/si/sl/bib/101899011 https://hdl.handle.net/20.500.12556/RUL-138602 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.9CBC3B5B |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |