التفاصيل البيبلوغرافية
| العنوان: |
ARHGEF26 and RHOG are context-dependent enhancers of IL-8 abundance in HeLa cell supernatant. |
| المؤلفون: |
Jeffrey S. Bourgeois (3129021), Liuyang Wang (3216039), Agustin F. Rabino (11106289), Jeffrey Everitt (11106292), Monica I. Alvarez (11106295), Sahezeel Awadia (11106298), Erika S. Wittchen (8087423), Rafael Garcia-Mata (183301), Dennis C. Ko (7147757) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Microbiology, Cell Biology, Genetics, Biotechnology, Immunology, Cancer, Infectious Diseases, Space Science, Biological Sciences not elsewhere classified, SopB, SopE 2-independent mechanism, GWAS, colitis infection model, invasion-associated host proteins, sopE 2 deletion, mice Salmonella hijack host machinery, enteric fever model, Typhimurium invasion, IL -8 production, ARHGEF 26, HeLa cells, SopE 2, enteric fever murine model, ARHGEF 26 stimulation, Arhgef 26 deletion, host cell type, ARHGEF 26-associated protein DLG 1, MDCK, Salmonella epithelial cell infection |
| الوصف: |
(A) ARHGEF26 and RHOG knockdown results in less IL-8 secretion into HeLa cell supernatant than non-targeting (NT) siRNA. Media was changed two days post transfection and supernatant was collected 8 hours later. (B) Overexpression of ARHGEF26 and RHOG in HeLa cells increases IL-8 cytokine abundance in supernatant. Media was changed on transfected cells 18–24 hours post transfection and supernatant was collected 6 hours later. (C, D) ARHGEF26 , but not RHOG , knockdown reduces IL-8 abundance in supernatant following S . Typhi infection (C) and S . Typhimurium infection (D). For C and D, cells were infected two days post transfection. Cells were infected with S . Typhi (MOI 30) or S . Typhimurium (MOI 1) for one hour before gentamicin addition. Media was changed two hours before infection and supernatant was collected six hours after infection. Cytokine abundance was measured by ELISA. For all graphs, dots represent a single well and data were collected across seven independent experiments. Data were normalized to the grand mean prior to plotting or performing statistics. For (C), two outliers identified by ROUT (Q = 0.1%) were removed from the non-targeting group. These values (17,924 pg/mL and 22,083 pg/mL) inflated the mean of the NT group, making the ARHGEF26 effect size artificially large, and the p-value artificially low (p = 0.002). P-values were calculated using a one-way ANOVA with Dunnett’s multiple comparison test on the log 2 transformed data. For all graphs central tendency is the mean, error bars are the standard error of the mean. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/ARHGEF26_and_RHOG_are_context-dependent_enhancers_of_IL-8_abundance_in_HeLa_cell_supernatant_/14944003 |
| DOI: |
10.1371/journal.ppat.1009713.g008 |
| الاتاحة: |
https://doi.org/10.1371/journal.ppat.1009713.g008 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.9CBB97B0 |
| قاعدة البيانات: |
BASE |