Academic Journal
Dendritic Cells and B Cells Cooperate in the Generation of CD4+ CD25+ FOXP3+ Allogeneic T Cells
| العنوان: | Dendritic Cells and B Cells Cooperate in the Generation of CD4+ CD25+ FOXP3+ Allogeneic T Cells |
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| المؤلفون: | Moore, C., Sauma Mahaluf, Daniela, Reyes Pérez, P. A., Morales Peña, José, Rosemblatt Silber, Mario César, Bono Merino, María Rosa, Fierro, J. A. |
| بيانات النشر: | ELSEVIER |
| سنة النشر: | 2010 |
| المجموعة: | Universidad de Chile: Repositorio académico |
| الوصف: | Background. CD4 CD25 Foxp3 regulatory T cells (Treg) play an essential role in immune tolerance, suppressing responses against self-antigens. Additionally, Treg play an important role in maintaining immunosuppression to alloantigens as well as to other antigens. It is well known that in the gut, a subset of dendritic cells produces retinoic acid (RA), which together with transforming growth factor (TGF- ) is able to differentiate naïve T cells into Treg. The aim of this study was to establish the role of antigen-presenting cells (APC) in the differentiation of allogeneic Tregs under the effect of RA and TGF- . Methods. Splenic CD4 CD25 naïve T cells from C57BL/6 mice were co-cultured with splenic CD11c-enriched APC from Balb/c mice in the presence of TGF- , RA, and interleukin (IL-2). After 6 days of culture, cells were analyzed for the expression of Foxp3 by flow cytometry. Additionally, we investigated the role of B cells and dendritic cells (DCs) and their stimulatory capacity in the generation of Tregs. Results. Our results showed that co-culture of naive T cells with the appropriate level of stimulation by APC in the presence of TGF- , RA, and IL-2 provided a new powerful approach to generate allogeneic Treg cells. We demonstrated that although B cells and DCs can generate Tregs by themselves, a mixure of both APC improved their capacity to efficiently generate Tregs. Also, we observed that although the addition of IL-2 to the cultures was not crucial to generate Tregs, it was required to optimize their expansion and cell survival. ; Supported by FONDECYT grants 1060834, 1060253, 1080416 Universidad Andres Bello grant DI-08-08/I and PFB-16 fron CONICYT, Chile. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | Transplantation Proceedings, 42, 371–375 (2010); http://repositorio.uchile.cl/handle/2250/119053 |
| DOI: | 10.1016/j.transproceed.2009.12.044 |
| الاتاحة: | https://doi.org/10.1016/j.transproceed.2009.12.044 http://repositorio.uchile.cl/handle/2250/119053 |
| رقم الانضمام: | edsbas.9C9F3225 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.transproceed.2009.12.044 |
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