Academic Journal
Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers
| العنوان: | Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers |
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| المؤلفون: | Martens, D E, Nollen, E A, Hardeveld, M, Velden-de Groot, C A, Gooijer, C D, Beuvery, E C, Tramper, J |
| المصدر: | Martens , D E , Nollen , E A , Hardeveld , M , Velden-de Groot , C A , Gooijer , C D , Beuvery , E C & Tramper , J 1997 , ' Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers ' , Cytotechnology , vol. 23 , no. 1 , pp. 61-75 . https://doi.org/10.1023/B:CYTO.0000010399.23373.65 |
| سنة النشر: | 1997 |
| المجموعة: | University of Groningen research database |
| مصطلحات موضوعية: | Journal Article |
| الوصف: | The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10(-3)m(3) liquid per m(3) of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10(-4)m(3) liquid per m(3) of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://research.rug.nl/en/publications/aa9e056b-2b6f-45c1-8145-e962cbc19b1a |
| DOI: | 10.1023/B:CYTO.0000010399.23373.65 |
| الاتاحة: | https://hdl.handle.net/11370/aa9e056b-2b6f-45c1-8145-e962cbc19b1a https://research.rug.nl/en/publications/aa9e056b-2b6f-45c1-8145-e962cbc19b1a https://doi.org/10.1023/B:CYTO.0000010399.23373.65 |
| Rights: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.9C4AB6C3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1023/B:CYTO.0000010399.23373.65 |
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