التفاصيل البيبلوغرافية
| العنوان: |
Hepatic xenobiotic metabolism of cylindrospermopsin in vivo in the mouse |
| المؤلفون: |
Norris, RLG, Seawright, AA, Shaw, GR, Senogles, P, Eaglesham, GK, Smith, MJ, Chiswell, RK, Moore, MR |
| بيانات النشر: |
Pergamon-Elsevier Science Ltd |
| سنة النشر: |
2002 |
| المجموعة: |
The University of Queensland: UQ eSpace |
| مصطلحات موضوعية: |
Pharmacology & Pharmacy, Toxicology, Mice, Cylindrospermopsin, Piperonyl Butoxide, Glutathione, P450, Cyanobacterial Alkaloid Cylindrospermopsin, Cultured Rat Hepatocytes, Glutathione Synthesis, Raciborskii Woloszynska, Drug-metabolism, Queensland, Toxicity, Cattle, 321299 Public Health and Health Services not elsewhere classified, C1, 730210 Environmental health |
| الوصف: |
Cylindrospermopsin (CYN) is a hepatotoxin isolated from the blue-green alga Cylindrospermopsis raciborskii. The role of both glutathione (GSH) and the cytochrome P450 enzyme system (P450) in the mechanism of toxicity of CYN has been previously investigated in in vitro systems. We have investigated the role of GSH and P450 in vivo in mice. Mice pre-treated with buthionine sulphoximine and diethyl maleate to deplete hepatic GSH prior to dosing with 0.2 mg/kg CYN showed a seven-day survival rate of 5/13 while the control group rate was 9/14. Dosing mice with 0.2 mg/kg CYN produced a small decrease in hepatic GSH with a characteristic rebound effect at 24 h, The magnitude of this effect is however small and combined with the non-significant difference in survival rates after GSH depletion suggest depletion of GSH by CYN could not be a primary mechanism for CYN toxicity, Conversely, pro-treatment with piperonyl butoxide, a P450 inhibitor, protected mice against CYN toxicity giving a survival rate of 10/10 compared with 4/10 in the control group (p < 0.05 Chi squared) and was protective at doses up to 0.8 mg/kg, suggesting activation of CYN by P450 is of primary importance in the mechanism of action. (C) 2002 Elsevier Science Ltd. All rights reserved. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| تدمد: |
0041-0101 |
| الاتاحة: |
https://espace.library.uq.edu.au/view/UQ:63424 |
| رقم الانضمام: |
edsbas.9C1076BC |
| قاعدة البيانات: |
BASE |