Academic Journal
Inhibition of chymotrypsin by heparin cofactor II.
| العنوان: | Inhibition of chymotrypsin by heparin cofactor II. |
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| المؤلفون: | Church, F. C., Noyes, C. M., Griffith, M. J. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America, 82(19) |
| سنة النشر: | 1985 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | Protéine sérique, Human, Homme, Hydrolase, Proteina serica, Cinetica, Hidrolasa, Inhibiteur enzyme, Enzima, Inhibition, Inhibicion, Hombre, Inhibidor enzima, Serum protein, Thrombin, Chymotrypsin, Enzyme, Cinétique, Héparine cofacteur 2, Enzyme inhibitor, Kinetics |
| الوصف: | Human heparin cofactor II is a plasma protein that is known to inhibit thrombin. The rate of thrombin inhibition by heparin cofactor II is accelerated (greater than or equal to 1000-fold) in the presence of the glycosaminoglycans, heparin and dermatan sulfate. We have found that chymotrypsin A alpha is also inhibited by heparin cofactor II with a second-order rate constant value of 1.8 X 10(6) M-1 X min-1 at pH 8.0 and 25 degrees C. However, there was no measurable effect of heparin or dermatan sulfate on the rate of chymotrypsin inhibition. Arginine-modified heparin cofactor II showed a comparable percentage loss of both antichymotrypsin and antithrombin activities. Heparin cofactor II and chymotrypsin formed a stable complex with a Mr value near 90,000 when analyzed by NaDodSO4/polyacrylamide gel electrophoresis; this suggests a 1:1 reaction stoichiometry. The chymotrypsin cleavage site in heparin cofactor II was the same as that for thrombin, and primary structure analysis of the inhibitor showed a P'1-P'8 sequence of Ser-Thr-Gln-Val-Arg-Phe-Thr-Val . . The results indicate that, in contrast to alpha 1-antichymotrypsin, which does not inhibit trypsin-like enzymes, including thrombin, heparin cofactor II can effectively inhibit both thrombin and chymotrypsin. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://doi.org/10.17615/952j-a077; https://cdr.lib.unc.edu/downloads/4f16c971p?file=thumbnail; https://cdr.lib.unc.edu/downloads/4f16c971p |
| DOI: | 10.17615/952j-a077 |
| الاتاحة: | https://doi.org/10.17615/952j-a077 https://cdr.lib.unc.edu/downloads/4f16c971p?file=thumbnail https://cdr.lib.unc.edu/downloads/4f16c971p |
| Rights: | http://rightsstatements.org/vocab/InC/1.0/ |
| رقم الانضمام: | edsbas.9BB1B20D |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/952j-a077 |
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