التفاصيل البيبلوغرافية
| العنوان: |
Cross-Species Proteomic Comparison of Outer Membrane Vesicles and Membranes of Francisella tularensis subsp. tularensis versus subsp. holarctica |
| المؤلفون: |
Jana Klimentova (2339959), Pavel Rehulka (1849525), Ivona Pavkova (2339947), Klara Kubelkova (641141), Jan Bavlovic (7482143), Jiri Stulik (151536) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Medicine, Microbiology, Cell Biology, Immunology, Infectious Diseases, expression changes, proteomic comparison, single-species databases, Francisella tularensis, membrane vesicles, quantification errors, tularensis strains, intersection protein database, holarctica Release, envelope components, membrane proteins, holarctica strain FSC 200, tularensis strain SchuS 4. Proteomi., cross-species proteomic comparison, Francisella tularensis subsp, protein amounts, OMV, Outer Membrane Vesicles, quantification issues, Gram-negative bacteria, novel approach, PXD, membrane fractions |
| الوصف: |
Release of outer membrane vesicles (OMV) is an important phenomenon in Gram-negative bacteria playing multiple roles in their lifestyle, including in relation to virulence and host–pathogen interaction. Francisella tularensis, unlike other bacteria, releases unusually shaped, tubular OMV. We present a proteomic comparison of OMV and membrane fractions from two F. tularensis strains: moderately virulent subsp. holarctica strain FSC200 and highly virulent subsp. tularensis strain SchuS4. Proteomic comparison studies routinely evaluate samples from the same proteome, but sometimes we must compare samples from closely related organisms. This raises quantification issues. We propose a novel approach to cross-species proteomic comparison based on an intersection protein database from the individual single-species databases. This is less prone to quantification errors arising from differences in the sequences. Consecutively comparing subproteomes of OMV and membranes of the two strains allows distinguishing differences in relative protein amounts caused by global expression changes from those caused by preferential protein packing to OMV or membranes. Among the proteins most differently packed into OMV between the two strains, we detected proteins involved in biosynthesis and metabolism of bacterial envelope components like O-antigen, lipid A, phospholipids, and fatty acids, as well as some major structural outer membrane proteins. The data are available via ProteomeXchange with identifier PXD022406. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Cross-Species_Proteomic_Comparison_of_Outer_Membrane_Vesicles_and_Membranes_of_Francisella_tularensis_subsp_i_tularensis_i_versus_subsp_i_holarctica_i_/13721326 |
| DOI: |
10.1021/acs.jproteome.0c00917.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.jproteome.0c00917.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.9B92E588 |
| قاعدة البيانات: |
BASE |