التفاصيل البيبلوغرافية
| العنوان: |
Mycobacterium avium subsp. paratuberculosis (MAP) in Goat Milk in the Semiarid Region of the Brazilian Northeast - Molecular Characterization ; A Caracterização molecular de Mycobacterium avium subespécie paratuberculosis (MAP) em leite de cabra destinado a usinas de processamento no semiárido do Nordeste brasileiro. |
| المؤلفون: |
Roberto, João Paulo de Lacerda, Limeira, Clécio Henrique, Rodrigues Soares, Rafael, Araújo Júnior, João Pessoa, Malossi, Camila Dantas, Ullmann, Leila Sabrina, Silva, Maria Luana Cristiny Rodrigues, Higino, Severino Silvano dos Santos, Azevedo, Sérgio Santos, Alves, Clebert José |
| المصدر: |
Acta Scientiae Veterinariae; Vol. 50 (2022): ARTICLES ; Acta Scientiae Veterinariae; v. 50 (2022): ARTICLES ; 1679-9216 |
| بيانات النشر: |
Universidade Federal do Rio Grande do Sul |
| سنة النشر: |
2022 |
| المجموعة: |
SEER UFRGS (Universidade Federal do Rio Grande do Sul: Sistema Eletrônico de Editoração de Periódic) |
| الوصف: |
Background: Goat farming has been on the rise in Brazil in recent years. Overall, 93% of the national herd is concentrated in the Northeast, with the state of Paraíba being the largest goat milk producer in the country. Considering Mycobacterium avium subsp. paratuberculosis (MAP) as a sanitary issue for the development of animal farming with risks for human health and that is a notifiable disease, this research was structured with the objective of confirming the presence and performing a molecular characterization of MAP in goat milk destined for processing plants in the semiarid region of the Brazilian Northeast. Materials, Methods & Results: Samples from 179 production units and 5 collective bulk tanks and 4 samples of pasteurized goat milk were analyzed through Real-time polymerase chain reaction (qPCR). Genetic material (DNA) for MAP was found in the goat milk sample from 1 production unit (1/179). From this positive sample, 9 lactating goats were identified in the original property, 7 of which showed MAP DNA in milk samples (77.77%). The characterization of the nucleotide sequence detected in the positive sample has 99% identity with KJ173784. Discussion: One sample (1/179), from the production units, had MAP genetic material (DNA) detected using the molecular test. Samples from these production units represent the milk from all lactating goats from each producer. Therefore, it was possible to identify from which farm the samples originated, allowing individual animals to then be tested, with milk samples collected from 9 goats and MAP DNA detected in 7 of them (77.77%) via PCR. Control and/or prevention programs need this type of surveillance in reason that it allows the tracking of possible foci from milk samples collected from dairy products or cooling stations. The use of PCR to detect MAP foci via goat milk is thus advantageous because samples are obtained in a non-invasive manner, with faster results when compared to the culture technique. The low detection via PCR in goat milk may be related to ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://seer.ufrgs.br/index.php/ActaScientiaeVeterinariae/article/view/125071/86762; https://seer.ufrgs.br/index.php/ActaScientiaeVeterinariae/article/view/125071 |
| الاتاحة: |
https://seer.ufrgs.br/index.php/ActaScientiaeVeterinariae/article/view/125071 |
| Rights: |
Copyright (c) 2022 João Paulo de Lacerda Roberto, Clécio Henrique Limeira, Rafael Rodrigues Soares, João Pessoa Araújo Júnior, Camila Dantas Malossi, Leila Sabrina Ullmann, Maria Luana Cristiny Rodrigues Silva, Severino Silvano dos Santos Higino, Sérgio Santos Azevedo, Clebert José Alves ; http://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: |
edsbas.9B7DD456 |
| قاعدة البيانات: |
BASE |