Academic Journal
The CD49d/CD29 complex is physically and functionally associated with CD38 in B-cell chronic lymphocytic leukemia cells
| العنوان: | The CD49d/CD29 complex is physically and functionally associated with CD38 in B-cell chronic lymphocytic leukemia cells |
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| المؤلفون: | A. Zucchetto, T. Vaisitti, D. Benedetti, E. Tissino, V. Bertagnolo, D. Rossi, R. Bomben, M. Dal Bo, M. I. Del Principe, A. Gorgone, G. Gaidano, G. Del Poeta, F. Malavasi, S. Deaglio, V. Gattei, POZZATO, GABRIELE |
| المساهمون: | A., Zucchetto, T., Vaisitti, D., Benedetti, E., Tissino, V., Bertagnolo, D., Rossi, R., Bomben, M., Dal Bo, M. I., Del Principe, A., Gorgone, Pozzato, Gabriele, G., Gaidano, G., Del Poeta, F., Malavasi, S., Deaglio, V., Gattei |
| سنة النشر: | 2012 |
| المجموعة: | Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste) |
| مصطلحات موضوعية: | CD38, CD49d, chronic lymphocytic leukemia, CLL, integrins |
| الوصف: | CD49d and CD38 are independent negative prognostic markers in chronic lymphocytic leukemia (CLL). Their associated expression marks a disease subset with a highly aggressive clinical course. Here, we demonstrate a constitutive physical association between the CD49d/CD29 integrin complex and CD38 in primary CLL cells and B-cell lines by (i) cocapping, (ii) coimmunoprecipitation and (iii) cell adhesion experiments using CD49d-specific substrates (vascular-cell adhesion molecule-1 or CS-1/H89 fibronectin fragments). The role of CD38 in CD49d-mediated cell adhesion was studied in CD49d CD38 and CD49d + CD38 - primary CLL cells, and confirmed using CD38 transfectants of the originally CD49d + CD38 - CLL-derived cell line Mec-1. Results indicate that CD49d + CD38 - cells adhered more efficiently onto CD49d-specific substrates than CD49d CD38 cells (P0.001). Upon adhesion, CD49d + CD38 - cells underwent distinctive changes in cell shape and morphology, with higher levels of phosphorylated Vav-1 than CD49d + CD38 - cells (P0.0006) and a more complex distribution of F-actin to the adhesion sites. Lastly, adherent CD49d + CD38 - cells were more resistant to serum-deprivation- induced (P0.001) and spontaneous (P0.03) apoptosis than the CD49d + CD38 -s counterpart. Altogether, our results point to a direct role for CD38 in enhancing CD49d-mediated adhesion processes in CLL, thus providing an explanation for the negative clinical impact exerted by these molecules when coexpressed in neoplastic cells. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/22289918; info:eu-repo/semantics/altIdentifier/wos/WOS:000305081000018; volume:26; issue:6; firstpage:1301; lastpage:1312; numberofpages:12; journal:LEUKEMIA; http://hdl.handle.net/11368/2855239; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84862016635; http://www.ncbi.nlm.nih.gov/pubmed/?term=22289918 |
| DOI: | 10.1038/leu.2011.369 |
| الاتاحة: | http://hdl.handle.net/11368/2855239 https://doi.org/10.1038/leu.2011.369 http://www.ncbi.nlm.nih.gov/pubmed/?term=22289918 |
| رقم الانضمام: | edsbas.9B379918 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/leu.2011.369 |
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