Academic Journal
Microarray-Based Identification of htrA , a Streptococcus pneumoniae Gene That Is Regulated by the CiaRH Two-Component System and Contributes to Nasopharyngeal Colonization
| العنوان: | Microarray-Based Identification of htrA , a Streptococcus pneumoniae Gene That Is Regulated by the CiaRH Two-Component System and Contributes to Nasopharyngeal Colonization |
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| المؤلفون: | Sebert, M. E., Palmer, L. M., Rosenberg, M., Weiser, J. N. |
| المصدر: | Infection and Immunity ; volume 70, issue 8, page 4059-4067 ; ISSN 0019-9567 1098-5522 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2002 |
| الوصف: | Nasopharyngeal carriage is the reservoir from which most disease with Streptococcus pneumoniae arises. Survival as a commensal in this environment is likely to require a set of adaptations distinct from those needed to cause disease, some of which may be mediated by two-component signal transduction systems (TCSTS). We examined the contributions of nine pneumococcal TCSTS to the process of nasopharyngeal colonization by using an infant rat model. Whereas deletions in all but one of these systems have been associated previously with a high degree of attenuation in a murine model of pneumonia, only the CiaRH system was necessary for efficient carriage. Transcriptional analysis by using microarray hybridization identified a locus consisting of two adjacent genes, htrA and spoJ , that was specifically and strongly downregulated in a Δ ciaRH -null mutant. A S. pneumoniae strain lacking the htrA gene encoding a putative serine protease, but not one lacking spoJ , showed decreased fitness in a competitive model of colonization, a finding consistent with this gene mediating a portion of the carriage deficit observed with the Δ ciaRH strain. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/iai.70.8.4059-4067.2002 |
| DOI: | 10.1128/IAI.70.8.4059-4067.2002 |
| الاتاحة: | http://dx.doi.org/10.1128/iai.70.8.4059-4067.2002 https://journals.asm.org/doi/pdf/10.1128/IAI.70.8.4059-4067.2002 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.9A0CA3E8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/iai.70.8.4059-4067.2002 |
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