Academic Journal
Multicentre evaluation of a selective isolation protocol for detection of mcr ‐positive E. coli and Salmonella spp. in food‐producing animals and meat
| العنوان: | Multicentre evaluation of a selective isolation protocol for detection of mcr ‐positive E. coli and Salmonella spp. in food‐producing animals and meat |
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| المؤلفون: | Perrin-Guyomard, Agnes, Granier, Sophie, Slettemeås, Jannice Schau, Anjum, Muna, Randall, Luke, Abuoun, Manal, Pauly, Natalie, Irrgang, Alexandra, Hammerl, Jens Andre, Kjeldgaard, Jette Sejer, Hammerum, Anette, Franco, Alessia, Skarżyńska, Magdalena, Kamińska, Ewelina, Wasyl, Dariusz, Dierikx, Cindy, Börjesson, Stefan, Geurts, Yvon, Haenni, Marisa, Veldman, Kees |
| المساهمون: | Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Norwegian Veterinary Institute Oslo, Animal and Plant Health Agency Addlestone, UK (APHA), German Federal Institute for Risk Assessment Berlin (BfR), Danmarks Tekniske Universitet = Technical University of Denmark (DTU), Statens Serum Institut Copenhagen, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana (IZSLT), National Veterinary Research Institute Pulawy, Pologne (NVRI), National Institute for Public Health and the Environment Bilthoven (RIVM), Public Health Agency of Sweden, Wageningen BioVeterinary Research, Wageningen University and Research Wageningen (WUR), Laboratoire de Lyon ANSES, Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), European Project: 773830, H2020-SFS-2017-1 ,One Health EJP(2018) |
| المصدر: | ISSN: 0266-8254. |
| بيانات النشر: | HAL CCSD Oxford University Press |
| سنة النشر: | 2022 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | microbiology, bacteria, Escherichia coli, Salmonella, food-borne pathogen, contaminant, food safety, detection, PCR, meat, animals, Colistin resistance, E. coli, mcr, screening, microbiologie, bactérie, Eschérichia coli, Salmonelle, pathogénes alimentaires, sécurité des aliments, détection, viande, MESH: Salmonella, MESH: Escherichia coli, MESH: Food Safety, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
| الوصف: | International audience ; This study was conducted to evaluate the performance of a screening protocol to detect and isolate mcr-positive Escherichia coli and Salmonella spp. from animal caecal content and meat samples. We used a multicentre approach involving 12 laboratories from nine European countries. All participants applied the same methodology combining a multiplex PCR performed on DNA extracted from a pre-enrichment step, followed by a selective culture step on three commercially available chromogenic agar plates. The test panel was composed of two negative samples and four samples artificially contaminated with E. coli and Salmonella spp. respectively harbouring mcr-1 or mcr-3 and mcr-4 or mcr-5 genes. PCR screening resulted in a specificity of 100% and a sensitivity of 83%. Sensitivity of each agar medium to detect mcr-positive colistin-resistant E. coli or Salmonella spp. strains was 86% for CHROMID® Colistin R, 75% for CHROMagarTM COL-APSE and 70% for COLISTIGRAM. This combined method was effective to detect and isolate most of the E. coli or Salmonella spp. strains harbouring different mcr genes from food-producing animals and food products and might thus be used as a harmonized protocol for the screening of mcr genes in food-producing animals and food products in Europe. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/35388505; info:eu-repo/grantAgreement//773830/EU/Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards./One Health EJP; anses-03654006; https://anses.hal.science/anses-03654006; https://anses.hal.science/anses-03654006/document; https://anses.hal.science/anses-03654006/file/Perrin_Colistin%20_LAM_%202022.pdf; PUBMED: 35388505; PUBMEDCENTRAL: PMC9544698 |
| DOI: | 10.1111/lam.13717 |
| الاتاحة: | https://anses.hal.science/anses-03654006 https://anses.hal.science/anses-03654006/document https://anses.hal.science/anses-03654006/file/Perrin_Colistin%20_LAM_%202022.pdf https://doi.org/10.1111/lam.13717 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.99F7E639 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/lam.13717 |
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