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Individual encasing of NSC lineages relies on strong intralineage adhesion through Nrg.
| العنوان: | Individual encasing of NSC lineages relies on strong intralineage adhesion through Nrg. |
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| المؤلفون: | Agata Banach-Latapy, Vincent Rincheval, David Briand, Isabelle Guénal, Pauline Spéder |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Cell Biology, Neuroscience, Developmental Biology, Cancer, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, stem cell population, shapes adult behaviour, div >< p, defined cellular microenvironment, individual stem cell, related cells regardless, neural stem cells, altered cg network, individual encasing, also altered, wrapper exists, weaker interaction, stereotypic structure, resulting adults, nsc lineages, newborn neurons, neuroglian results, membranous chambers, mechanisms building, locomotor hyperactivity, larval brain, intralineage adhesion, functional relevance |
| الوصف: | ( A ) Schematic depicting the 2 isoforms for Nrg, Nrg 167 , and Nrg 180 . Only the intracellular C-terminal part differs and will modulate downstream signalling. ( B ) Representative confocal close-up picture of the localisation of the Nrg 180 isoform in NSC lineages, in a thoracic VNC at ALH72 at 25°C. n = 8 VNCs. All Nrg isoforms are monitored through a Nrg :: GFP protein trap (magenta) and the Nrg 180 isoform is detected with a specific antibody (BP104, light blue). ( C ) Representative confocal close-up picture of the respective localisations of the Nrg 167 and Nrg 180 isoforms in NSC lineages, in a thoracic VNC at ALH72 at 25°C. n = 6 VNCs. The Nrg 167 isoform is visualised by a protein trap in the nrg gene leading to the preferential expression of this isoform ( Nrg 167 :: GFP , yellow). The Nrg 180 isoform is detected with a specific antibody (BP104, light blue). The dashed white line highlights the perimeter of the NSC devoid of BP104 signal. ( D ) Representative confocal picture of a thoracic VNC and close-up for nrg 180 overexpression in the CG from ALH0 ( cyp > nrg 180 , driver Nrv2 :: GFP , tub-GAL80 ts ; cyp4g15-GAL4 ). Larvae are dissected after 68 h at 29°C. CG membrane is visualised by Nrv2 :: GFP (green), NSCs are labelled with an anti-Dpn (grey), and Nrg 180 is detected with a specific antibody (BP104, magenta). ( E ) Representative confocal pictures of thoracic VNCs for nrg 167 and nrg GP I overexpression in the CG from ALH0 ( cyp > nrg 167 and cyp > nrg GPI , respectively, driver Nrv2 :: GFP , tub-GAL80 ts ; cyp4g15-GAL4 ). Larvae are dissected after 68 h at 29°C. CG membrane is visualised by Nrv2 :: GFP (green), and NSCs are labelled with an anti-Dpn (grey). ( F ) Quantification of the percentage of NSCs non-individually encased from ( D , E ). Control, cyp >— (x w 1118 ) ( n = 10 VNCs), cyp > nrg 180 ( n = 11 VNCs), cyp > nrg 167 ( n = 7 VNCs), and cyp > nrg GPI ( n = 6 VNCs). Data statistics: generalised linear model (Binomial regression with a Bernoulli distribution) ... |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/Individual_encasing_of_NSC_lineages_relies_on_strong_intralineage_adhesion_through_Nrg_/24537916 |
| DOI: | 10.1371/journal.pbio.3002352.g009 |
| الاتاحة: | https://doi.org/10.1371/journal.pbio.3002352.g009 https://figshare.com/articles/figure/Individual_encasing_of_NSC_lineages_relies_on_strong_intralineage_adhesion_through_Nrg_/24537916 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.999F9D5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pbio.3002352.g009 |
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