Academic Journal
Simultaneous Determination of Thirteen Iridoid Glycosides in Crude and Processed Fructus Corni from Different Areas by UPLC–MS/MS Method
| العنوان: | Simultaneous Determination of Thirteen Iridoid Glycosides in Crude and Processed Fructus Corni from Different Areas by UPLC–MS/MS Method |
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| المؤلفون: | Zhang, Fan, Yan, Yu, Xu, Jie-Kun, Zhang, Lin-Mei, Li, Li, Chen, Xin, Li, Dong-Xu, Peng, Ying, Yang, Hua, Li, Ling-Zhi, He, Jun, Zheng, Jiang, Zhang, Wei-Ku |
| المساهمون: | Open Research Fund Program of China National Light Industry Key Laboratory of Cosmetic, Beijing Technology and Business University, Open Project of the State Key Laboratory of Natural Medicine Active Substances and Functions, Chinese Pharmaceutical Association-Yiling Biomedical Innovation Fund Project, Yan'an Science and Techohogy Plan Project, The Nonprofit Central Research Institute Fund of Chinese Academy of Medical Sciences, National Natural Science Foundation of China, Elite Medical Professionals Project of China-Japan Friendship Hospital, National High Level Hospital Clinical Research Funding |
| المصدر: | Journal of Chromatographic Science ; volume 62, issue 6, page 562-569 ; ISSN 0021-9665 1945-239X |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2023 |
| الوصف: | Fructus Corni (F. Corni) is the dried mature pulp of Cornus officinalis Sieb. et Zucc.(Cornaceae), which is rich in iridoids. In this study, a simple, sensitive and rapid UPLC–MS/MS method was developed for the simultaneous determination of 13 iridoid glycosides of F. Corni from different areas. Specifically, we included five new compounds (cornusdiridoid C, cornusdiridoid E, cornusdiridoid F, 3′′,5′′-dehydroxycornuside and 2′-O-p-coumaroyl-kingiside) and isomers (2′-O-p-E-coumaroylloganin and 2′-O-p-Z-coumaroylloganin) for the first time in the quality markers of F. Corni. A total of 13 compounds and two pairs of isomers were well isolated and tested within just 14 min. All calibration curves showed good linear regression (r2 ≥ 0.99) within the tested concentration ranges. The limit of detection and limit of quantification were in the range of 0.19–1.90 and 0.38–3.76 ng/mL, respectively. The intra-day and inter-day precision were <3.21% and 12.49%, the RSD values of repeatability did not exceed 6.81% and the average recoveries were 90.95–113.59% for the analytes. All iridoid glycosides stabilized within 12 h (RSD < 10.99%). This method has been successfully applied to the quality evaluation of crude and processed F. Corni from different areas. The determination of characteristic iridoid glycosides and isomers will provide a more reliable and comprehensive method for the evaluation of F. Corni. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/chromsci/bmad083 |
| الاتاحة: | http://dx.doi.org/10.1093/chromsci/bmad083 https://academic.oup.com/chromsci/article-pdf/62/6/562/58496231/bmad083.pdf |
| Rights: | https://academic.oup.com/pages/standard-publication-reuse-rights |
| رقم الانضمام: | edsbas.9994125A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/chromsci/bmad083 |
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