Academic Journal
Synthesis of Novel Products In Vitro by an
| العنوان: | Synthesis of Novel Products In Vitro by an |
|---|---|
| المؤلفون: | Rna-dependent Rna Polymerase, Chuanzheng Song, Anne, E. Simon |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://www.life.umd.edu/CBMG/faculty/asimon/Journal/J Virology 1995.pdf. |
| سنة النشر: | 1994 |
| المجموعة: | CiteSeerX |
| الوصف: | RNA-dependent RNA polymerase from turnip crinkle virus-infected turnip transcribes both strands of a virus-associated satellite RNA, sat-RNA C (356 bases), in vitro. While both plus- and minus-strand sat-RNA C can direct the synthesis of full-length complementary-strand products, transcription of minus-strand RNA also generates two non-template-sized products, L-RNA and S-RNA (C. Song and A. E. Simon, Proc. Natl. Acad. Sci. USA 91:8792–8796, 1994). Here we report that synthesis of L-RNA and S-RNA results from terminal elongation of the 3 * end of the template. L-RNA has a panhandle structure and is composed of minus-strand template covalently linked to newly synthesized RNA complementary to its 5 * 190 bases. S-RNA is composed of template covalently linked to its full-length complementary strand. All minus-strand templates tested yielded S-RNA. However, synthesis of L-RNA was affected by deletion of the 3 * end of the minus-strand template or several internal regions and base alterations near the 5 * end or in an internal sequence immedi-ately upstream from the template-product junction that could potentially form a heteroduplex with the 3 * end. Furthermore, mutations that disrupted or restored a stem-loop involved in RNA recombination in vivo affected the level of L-RNA produced in vitro, suggesting that the mechanisms for intramolecular formation of panhandle RNAs and intermolecular RNA recombination involve similar features. Positive-strand RNA viruses rely on RNA-dependent RNA |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.583.9633; http://www.life.umd.edu/CBMG/faculty/asimon/Journal/J Virology 1995.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.583.9633 http://www.life.umd.edu/CBMG/faculty/asimon/Journal/J Virology 1995.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.997EEB0A |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |