التفاصيل البيبلوغرافية
| العنوان: |
Ultrahigh-Resolution NMR with Water Signal Suppression for a Deeper Understanding of the Action of Antimetabolic Drugs on Diffuse Large B‑Cell Lymphoma |
| المؤلفون: |
Gildas Bertho (1844320), Leonardo Lordello (12047688), Xi Chen (35903), Covadonga Lucas-Torres (5919119), Imed Eddine Oumezziane (12047691), Cédric Caradeuc (12047694), Mathieu Baudin (8697579), Stéphanie Nuan-Aliman (12047697), Catherine Thieblemont (282172), Véronique Baud (3635827), Nicolas Giraud (1777699) |
| سنة النشر: |
2022 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Genetics, Biotechnology, Cancer, Plant Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, statistical analyses carried, pure shift methods, pure shift data, greatly simplify crowded, diffuse large b, highlighting metabolite mixtures, resolution 1d noesy, complex samples analysis, 1 , dlbcl cell line, water signal suppression, water signal, metabolomics analysis, deciphering mixtures, cell lymphoma, biological samples, yielding singlets, successfully implemented, standard approach, solvent suppression |
| الوصف: |
Ultrahigh-resolution NMR has recently attracted considerable attention in the field of complex samples analysis. Indeed, the implementation of broadband homonuclear decoupling techniques has allowed us to greatly simplify crowded 1 H spectra, yielding singlets for almost every proton site from the analyzed molecules. Pure shift methods have notably shown to be particularly suitable for deciphering mixtures of metabolites in biological samples. Here, we have successfully implemented a new pure shift pulse sequence based on the PSYCHE method, which incorporates a block for solvent suppression that is suitable for metabolomics analysis. The resulting experiment allows us to record ultrahigh-resolution 1D NOESY 1 H spectra of biofluids with suppression of the water signal, which is a crucial step for highlighting metabolite mixtures in an aqueous phase. We have successfully recorded pure shift spectra on extracellular media of diffuse large B-cell lymphoma (DLBCL) cells. Despite a lower sensitivity, the resolution of pure shift data was found to be better than that of the standard approach, which provides a more detailed vision of the exo-metabolome. The statistical analyses carried out on the resulting metabolic profiles allow us to successfully highlight several metabolic pathways affected by these drugs. Notably, we show that Kidrolase plays a major role in the metabolic pathways of this DLBCL cell line. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Ultrahigh-Resolution_NMR_with_Water_Signal_Suppression_for_a_Deeper_Understanding_of_the_Action_of_Antimetabolic_Drugs_on_Diffuse_Large_B_Cell_Lymphoma/19125486 |
| DOI: |
10.1021/acs.jproteome.1c00914.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.jproteome.1c00914.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.99793083 |
| قاعدة البيانات: |
BASE |