التفاصيل البيبلوغرافية
| العنوان: |
Profiling of lung SARS-CoV-2 and influenza virus infection dissects virus-specific host responses and gene signatures |
| المؤلفون: |
Kulasinghe, Arutha, Tan, Chin Wee, Ribeiro Dos Santos Miggiolaro, Anna Flavia, Monkman, James, SadeghiRad, Habib, Bhuva, Dharmesh D, Motta Junior, Jarbas da Silva, Busatta Vaz de Paula, Caroline, Nagashima, Seigo, Baena, Cristina Pellegrino, Souza-Fonseca-Guimaraes, Paulo, de Noronha, Lucia, McCulloch, Timothy, Rossi, Gustavo Rodrigues, Cooper, Caroline, Tang, Benjamin, Short, Kirsty R, Davis, Melissa J, Souza-Fonseca-Guimaraes, Fernando, Belz, Gabrielle T, O'Byrne, Ken |
| المصدر: |
The European Respiratory Journal |
| بيانات النشر: |
European Respiratory Society |
| سنة النشر: |
2022 |
| المجموعة: |
Queensland University of Technology: QUT ePrints |
| مصطلحات موضوعية: |
COVID-19/genetics, Humans, Influenza A Virus, H1N1 Subtype, Influenza, Human/genetics, Interferon Type I/metabolism, Lung/pathology, SARS-CoV-2 |
| الوصف: |
BACKGROUND: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which emerged in late 2019 has spread globally, causing a pandemic of respiratory illness designated coronavirus disease 2019 (COVID-19). A better definition of the pulmonary host response to SARS-CoV-2 infection is required to understand viral pathogenesis and to validate putative COVID-19 biomarkers that have been proposed in clinical studies. METHODS: Here, we use targeted transcriptomics of formalin-fixed paraffin-embedded tissue using the NanoString GeoMX platform to generate an in-depth picture of the pulmonary transcriptional landscape of COVID-19, pandemic H1N1 influenza and uninfected control patients. RESULTS: Host transcriptomics showed a significant upregulation of genes associated with inflammation, type I interferon production, coagulation and angiogenesis in the lungs of COVID-19 patients compared to non-infected controls. SARS-CoV-2 was non-uniformly distributed in lungs (emphasising the advantages of spatial transcriptomics) with the areas of high viral load associated with an increased type I interferon response. Once the dominant cell type present in the sample, within patient correlations and patient-patient variation, had been controlled for, only a very limited number of genes were differentially expressed between the lungs of fatal influenza and COVID-19 patients. Strikingly, the interferon-associated gene IFI27, previously identified as a useful blood biomarker to differentiate bacterial and viral lung infections, was significantly upregulated in the lungs of COVID-19 patients compared to patients with influenza. CONCLUSION: Collectively, these data demonstrate that spatial transcriptomics is a powerful tool to identify novel gene signatures within tissues, offering new insights into the pathogenesis of SARS-COV-2 to aid in patient triage and treatment. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| Relation: |
https://eprints.qut.edu.au/233056/1/2101881.full.pdf; Kulasinghe, Arutha, Tan, Chin Wee, Ribeiro Dos Santos Miggiolaro, Anna Flavia, Monkman, James, SadeghiRad, Habib, Bhuva, Dharmesh D, Motta Junior, Jarbas da Silva, Busatta Vaz de Paula, Caroline, Nagashima, Seigo, Baena, Cristina Pellegrino, Souza-Fonseca-Guimaraes, Paulo, de Noronha, Lucia, McCulloch, Timothy, Rossi, Gustavo Rodrigues, Cooper, Caroline, Tang, Benjamin, Short, Kirsty R, Davis, Melissa J, Souza-Fonseca-Guimaraes, Fernando, Belz, Gabrielle T, & O'Byrne, Ken (2022) Profiling of lung SARS-CoV-2 and influenza virus infection dissects virus-specific host responses and gene signatures. The European Respiratory Journal, 59(6), Article number: 2101881.; http://purl.org/au-research/grants/nhmrc/1157741; https://eprints.qut.edu.au/233056/; Centre for Genomics and Personalised Health; Faculty of Health; School of Biomedical Sciences |
| الاتاحة: |
https://eprints.qut.edu.au/233056/ |
| Rights: |
free_to_read ; http://creativecommons.org/licenses/by-nc/4.0/ ; The authors 2022 ; This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
| رقم الانضمام: |
edsbas.995BE784 |
| قاعدة البيانات: |
BASE |