التفاصيل البيبلوغرافية
| العنوان: |
The assessment of Pseudomonas aeruginosa lectin LecA binding characteristics of divalent galactosides using multiple techniques |
| المؤلفون: |
Zaree, Pouya, Torano, Javier Sastre, de Haan, Cornelis A M, Scheltma, Richard A, Barendregt, Arjan, Thijssen, Vito, Yu, Guangyun, Flesch, Frits, Pieters, Roland J |
| المساهمون: |
Afd Chemical Biology and Drug Discovery, Virologie, dI&I I&I-1, Afd Biomol.Mass Spect. and Proteomics, Sub Biomol.Mass Spectrometry & Proteom., Chemical Biology and Drug Discovery, Biomolecular Mass Spectrometry and Proteomics |
| سنة النشر: |
2021 |
| مصطلحات موضوعية: |
binding kinetics, LecA inhibition, multivalency, protein–carbohydrate interactions, residence time |
| الوصف: |
Pseudomonas aeruginosa is a widespread opportunistic pathogen that is capable of colonizing various human tissues and is resistant to many antibiotics. LecA is a galactose binding tetrameric lectin involved in adhesion, infection and biofilm formation. This study reports on the binding characteristics of mono- and divalent (chelating) ligands to LecA using different techniques. These techniques include Affinity Capillary Electrophoresis (ACE), Bio Layer Interferometry (BLI), Native Mass Spectrometry and a Thermal Shift Assay. Aspects of focus include: affinity, selectivity, binding kinetics and residence time. The affinity of a divalent ligand was determined to be in the low nanomolar range for all of the used techniques and with a ligand residence time of approximately 7 hours, while no strong binding was seen to related lectin tetramers. Each of the used techniques provides a unique and complementary insight into the chelation based binding mode of the divalent ligand to the LecA tetramer. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
0959-6658 |
| Relation: |
https://dspace.library.uu.nl/handle/1874/412720 |
| الاتاحة: |
https://dspace.library.uu.nl/handle/1874/412720 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: |
edsbas.9959F95E |
| قاعدة البيانات: |
BASE |
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