Academic Journal
A Fluorescent‐Labeled Phosphono Bisbenzguanidine As an Activity‐Based Probe for Matriptase
| العنوان: | A Fluorescent‐Labeled Phosphono Bisbenzguanidine As an Activity‐Based Probe for Matriptase |
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| المؤلفون: | Häußler, Daniela, Schulz‐Fincke, Anna‐Christina, Beckmann, Anna‐Madeleine, Keils, Aline, Gilberg, Erik, Mangold, Martin, Bajorath, Jürgen, Stirnberg, Marit, Steinmetzer, Torsten, Gütschow, Michael |
| المصدر: | Chemistry – A European Journal ; volume 23, issue 22, page 5205-5209 ; ISSN 0947-6539 1521-3765 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2017 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Activity‐based probes are compounds that exclusively form covalent bonds with active enzymes. They can be utilized to profile enzyme activities in vivo, to identify target enzymes and to characterize their function. The design of a new activity‐based probe for matriptase, a member of the type II transmembrane serine proteases, is based on linker‐connected bis‐benzguanidines. An amino acid, introduced as linker, bears the coumarin fluorophore. Moreover, an incorporated phosphonate allows for a covalent interaction with the active‐site serine. The resulting irreversible mode of action was demonstrated, leading to enzyme inactivation and, simultaneously, to a fluorescence labeling of matriptase. The ten‐step synthetic approach to a coumarin‐labeled bis‐benzguanidine and its evaluation as activity‐based probe for matriptase based on in‐gel fluorescence and fluorescence HPLC is reported. HPLC fluorescence detection as a new application for activity‐based probes for proteases is demonstrated herein for the first time. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/chem.201700319 |
| DOI: | 10.1002/chem.201700319/fullpdf |
| الاتاحة: | http://dx.doi.org/10.1002/chem.201700319 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fchem.201700319 http://onlinelibrary.wiley.com/wol1/doi/10.1002/chem.201700319/fullpdf |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.99574C0D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/chem.201700319 |
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