Academic Journal
BMP-3 PROMOTES MESENCHYMAL STEM CELL PROLIFERATION VIA THE TGF-ß/ACTIVIN PATHWAY
| العنوان: | BMP-3 PROMOTES MESENCHYMAL STEM CELL PROLIFERATION VIA THE TGF-ß/ACTIVIN PATHWAY |
|---|---|
| المؤلفون: | Stewart, Aaron Noelle |
| المصدر: | Digitized Theses |
| بيانات النشر: | Scholarship@Western |
| سنة النشر: | 2009 |
| المجموعة: | The University of Western Ontario: Scholarship@Western |
| مصطلحات موضوعية: | adipogenesis, adipocytes, preadipocytes, self-renewal, p38 MAPK, ERK1/2, JNK, Smad2, C3H10T1/2, 3T3-L1, [3H]-thymidine, SB-431542, SB-202190, U0126 |
| الوصف: | This study was designed to test the hypothesis that bone morphogenetic protein-3 (BMP-3) promotes adipogenesis using C3H10T1/2 mesenchymal stem cells (MSCs) and 3T3-L1 preadipocytes as in vitro model systems. Although having no effects on the commitment of C3H10T1/2 cells to the adipocyte lineage or the differentiation of 3T3-L1 preadipocytes to adipocytes, BMP-3 stimulated DNA synthesis (as determined by [3H]- thymidine incorporation) in both cell types. Furthermore, the mitogenic effects of BMP-3 were mediated by the TGF-p/activin signalling pathway, because BMP-3 activated the TGF-p/activin pathway in C3H10T1/2 cells, and the TGF-p/activin pathway inhibitor SB- 431542 blocked BMP-3-stimulated proliferation. Additionally, inhibitors of ERK1/2 and p38 MAPK pathways reduced proliferation under basal conditions. In conclusion, the present study demonstrates that BMP-3 stimulates proliferation of MSCs and preadipocytes in vitro, which provides a source for adipogenesis. Consequently, aberrant BMP-3 expression may have consequences not only for normal adipose tissue growth but also for the pathogenesis of obesity. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | https://ir.lib.uwo.ca/digitizedtheses/3764; https://ir.lib.uwo.ca/context/digitizedtheses/article/7583/viewcontent/2023_01_02_BMP_PROMOTES_MSC_PROLIFERATION_OCR.pdf |
| الاتاحة: | https://ir.lib.uwo.ca/digitizedtheses/3764 https://ir.lib.uwo.ca/context/digitizedtheses/article/7583/viewcontent/2023_01_02_BMP_PROMOTES_MSC_PROLIFERATION_OCR.pdf |
| رقم الانضمام: | edsbas.9955A12B |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |