Academic Journal
Photodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells
| العنوان: | Photodynamic therapy with the dual-mode association of IR780 to PEG-PLA nanocapsules and the effects on human breast cancer cells |
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| المؤلفون: | Marina Guimarães Carvalho Machado, Maria Alice de Oliveira, Elisa Gomes Lanna, Raoni Pais Siqueira, Gwenaelle Pound-Lana, Renata Tupinambá Branquinho, Vanessa Carla Furtado Mosqueira |
| المصدر: | Biomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112464- (2022) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Phototheranostics, IR780, Polymeric nanocapsules, Image-guided therapy, Photodynamic therapy, Polymer conjugation, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | IR780 is a near-infrared fluorescent dye, which can be applied as a photosensitizer in photodynamic (PDT) and photothermal (PTT) therapies and as a biodistribution tracer in imaging techniques. We investigated the growth and migration inhibition and mechanism of death of breast tumor cells, MCF-7 and MDA-MB-231, exposed to polymeric nanocapsules (NC) comprising IR780 covalently linked to the biodegradable polymer PLA (IR-PLA) and IR780 physically encapsulated (IR780-NC) in vitro. Both types of NC had mean diameters around 120 nm and zeta potentials around −40 mV. IR-PLA-NC was less cytotoxic than IR780 NC to a non-tumorigenic mammary epithelial cell line, MCF-10A, which is an important aspect of selectivity. Free-IR780 was more cytotoxic than IR-PLA-NC for MCF-7 and MDA-MB-231 cells after illumination with a 808 nm laser. IR-PLA NC was effective to inhibit colony formation (50%) and migration (30–40%) for both cancer cell lines. MDA-MB-231 cells were less sensitive to all IR780 formulations compared to MCF-7 cells. Cell uptake was higher with IR-PLA-NC than with IR780-NC and free-IR780 in both cancer cell lines (p < 0.05). NC uptake was higher in MCF-7 than in MDA-MB-231 cells. IR-PLA-NC induced a higher percentage of apoptosis upon illumination in MDA-MB-231 than in MCF-7 cells. The necrosis mechanism of death predominated in treatments with free-IR780 and with encapsulated IR780 NC, suggestive of damages at the plasma membrane. IR780 conjugated with PLA increased the apoptotic pathway and demonstrated potential as a multifunctional theranostic agent for breast cancer treatment with increased cellular uptake, photodynamic activity and more reliable tracking in cell-image studies. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-7533-3222-1 0-7533-3222-1 |
| تدمد: | 0753-3322 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0753332221012506; https://doaj.org/toc/0753-3322; https://doaj.org/article/34cc528a417d4fe3950c745309b8b63e |
| DOI: | 10.1016/j.biopha.2021.112464 |
| الاتاحة: | https://doi.org/10.1016/j.biopha.2021.112464 https://doaj.org/article/34cc528a417d4fe3950c745309b8b63e |
| رقم الانضمام: | edsbas.994C13 |
| قاعدة البيانات: | BASE |
Full Text Finder | ردمك: | 9780753332221 0753332221 |
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| تدمد: | 07533322 |
| DOI: | 10.1016/j.biopha.2021.112464 |