Academic Journal
Lissajous Scanning Two-photon Endomicroscope for In vivo Tissue Imaging
| العنوان: | Lissajous Scanning Two-photon Endomicroscope for In vivo Tissue Imaging |
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| المؤلفون: | Kim, Daniel Youngsuk, Hwang, Kyungmin, Ahn, Jinhyo, Seo, Yeong-Hyeon, Kim, Jae-Beom, Lee, Soyoung, Yoon, Jin-Hui, Kong, Eunji, Jeong, Yong, Jon, Sangyong, Kim, Pilhan, Jeong, Ki-Hun |
| المصدر: | Scientific Reports ; volume 9, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2019 |
| الوصف: | An endomicroscope opens new frontiers of non-invasive biopsy for in vivo imaging applications. Here we report two-photon laser scanning endomicroscope for in vivo cellular and tissue imaging using a Lissajous fiber scanner. The fiber scanner consists of a piezoelectric (PZT) tube, a single double-clad fiber (DCF) with high fluorescence collection, and a micro-tethered-silicon-oscillator (MTSO) for the separation of biaxial resonant scanning frequencies. The endomicroscopic imaging exhibits 5 frames/s with 99% in scanning density by using the selection rule of scanning frequencies. The endomicroscopic scanner was compactly packaged within a stainless tube of 2.6 mm in diameter with a high NA gradient-index (GRIN) lens, which can be easily inserted into the working channel of a conventional laparoscope. The lateral and axial resolutions of the endomicroscope are 0.70 µm and 7.6 μm, respectively. Two-photon fluorescence images of a stained kidney section and miscellaneous ex vivo and in vivo organs from wild type and green fluorescent protein transgenic (GFP-TG) mice were successfully obtained by using the endomicroscope. The endomicroscope also obtained label free images including autofluorescence and second-harmonic generation of an ear tissue of Thy1-GCaMP6 (GP5.17) mouse. The Lissajous scanning two-photon endomicroscope can provide a compact handheld platform for in vivo tissue imaging or optical biopsy applications. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41598-019-38762-w |
| الاتاحة: | http://dx.doi.org/10.1038/s41598-019-38762-w https://www.nature.com/articles/s41598-019-38762-w.pdf https://www.nature.com/articles/s41598-019-38762-w |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.9946F206 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-019-38762-w |
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