Academic Journal
Isotopomeric Peak Assignment for N 2 O in Cross-Labeling Experiments by Fiber-Enhanced Raman Multigas Spectroscopy
| العنوان: | Isotopomeric Peak Assignment for N 2 O in Cross-Labeling Experiments by Fiber-Enhanced Raman Multigas Spectroscopy |
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| المؤلفون: | Annika Blohm, Christian Domes, Torsten Frosch |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Ecology, Sociology, Plant Biology, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, various isotopomer peaks, thus plant growth, presented results indicate, isotopomeric peak assignment, enhanced raman spectroscopy, 18 , 17 , 16 , 15 , 14 , specific molecular vibrations, main processes responsible, improve nitrogen availability, 2 , processes producing n, isotopomers alongside n, specific measurements, nitrogen fertilizer, nitrogen cycle, total n, successful regulation, sensitive quantification, sensitive detection, multigas compositions |
| الوصف: | Human intervention in nature, especially fertilization, greatly increased the amount of N 2 O emission. While nitrogen fertilizer is used to improve nitrogen availability and thus plant growth, one negative side effect is the increased emission of N 2 O. Successful regulation and optimization strategies require detailed knowledge of the processes producing N 2 O in soil. Nitrification and denitrification, the main processes responsible for N 2 O emissions, can be differentiated using isotopic analysis of N 2 O. The interplay between these processes is complex, and studies to unravel the different contributions require isotopic cross-labeling and analytical techniques that enable tracking of the labeled compounds. Fiber-enhanced Raman spectroscopy (FERS) was exploited for sensitive quantification of N 2 O isotopomers alongside N 2 , O 2 , and CO 2 in multigas compositions and in cross-labeling experiments. FERS enabled the selective and sensitive detection of specific molecular vibrations that could be assigned to various isotopomer peaks. The isotopomers 14 N 15 N 16 O (2177 cm –1 ) and 15 N 14 N 16 O (2202 cm –1 ) could be clearly distinguished, allowing site-specific measurements. Also, isotopomers containing different oxygen isotopes, such as 14 N 14 N 17 O, 14 N 14 N 18 O, 15 N 15 N 16 O, and 15 N 14 N 18 O could be identified. A cross-labeling showed the capability of FERS to disentangle the contributions of nitrification and denitrification to the total N 2 O fluxes while quantifying the total sample headspace composition. Overall, the presented results indicate the potential of FERS for isotopic studies of N 2 O, which could provide a deeper understanding of the different pathways of the nitrogen cycle. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/Isotopomeric_Peak_Assignment_for_N_sub_2_sub_O_in_Cross-Labeling_Experiments_by_Fiber-Enhanced_Raman_Multigas_Spectroscopy/25146080 |
| DOI: | 10.1021/acs.analchem.3c04236.s001 |
| الاتاحة: | https://doi.org/10.1021/acs.analchem.3c04236.s001 https://figshare.com/articles/journal_contribution/Isotopomeric_Peak_Assignment_for_N_sub_2_sub_O_in_Cross-Labeling_Experiments_by_Fiber-Enhanced_Raman_Multigas_Spectroscopy/25146080 |
| Rights: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.9932EF1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.analchem.3c04236.s001 |
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