Academic Journal
Nasal carriage of Staphylococcus aureus in healthy dairy cows in Algeria: antibiotic resistance, enterotoxin genes and biofilm formation
| العنوان: | Nasal carriage of Staphylococcus aureus in healthy dairy cows in Algeria: antibiotic resistance, enterotoxin genes and biofilm formation |
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| المؤلفون: | Titouche, Yacine, Akkou, Madjid, Djaoui, Yasmina, Mechoub, Donia, Fatihi, Abdelhak, Campaña-Burguet, Allelen, Bouchez, Pascal, Bouhier, Laurence, Houali, Karim, Torres, Carmen, Nia, Yacine, Hennekinne, Jacques-Antoine |
| المساهمون: | Université Mouloud Mammeri Tizi Ouzou = Mouloud Mammeri University of Tizi-Ouzou (UMMTO), Université Saâd Dahlab Blida 1 (UB1), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Universidad de La Rioja (UR), Staphylocoques, Bacillus, Clostridies, Lait (SBCL), Laboratoire de sécurité des aliments, sites de Maisons-Alfort et de Boulogne-sur-Mer (LSAl), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire de sécurité des aliments de Maisons-Alfort (LSAl), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), project PID2022-139591OB-I00 financed by MCIN/AEI/10.13039/501100011033/FEDER, UE/The experimental work performed in the University of La Rioja has been performed with project PID2022-139591OB-I00 |
| المصدر: | ISSN: 1746-6148 ; BMC Veterinary Research ; https://hal.science/hal-04607821 ; BMC Veterinary Research, 2024, 20 (1), pp.247. ⟨10.1186/s12917-024-04103-x⟩. |
| بيانات النشر: | CCSD BioMed Central |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | S. aureus, Antibiotic susceptibility, Enterotoxin genes, Healthy cows, Nasal carriage, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Background Staphylococcus aureus can colonize and infect a variety of animal species. In dairy herds, it is one of the leading causes of mastitis cases. The objective of this study was to characterize the S . aureus isolates recovered from nasal swabs of 249 healthy cows and 21 breeders of 21 dairy farms located in two provinces of Algeria (Tizi Ouzou and Bouira). Methods The detection of enterotoxin genes was investigated by multiplex PCRs. Resistance of recovered isolates to 8 antimicrobial agents was determined by disc-diffusion method. The slime production and biofilm formation of S . aureus isolates were assessed using congo-red agar (CRA) and microtiter-plate assay. Molecular characterization of selected isolates was carried out by spa -typing and Multi-Locus-Sequence-Typing (MLST). Results S . aureus was detected in 30/249 (12%) and 6/13 (28.6%) of nasal swabs in cows and breeders, respectively, and a total of 72 isolates were recovered from positive samples (59 isolates from cows and 13 from breeders). Twenty-six of these isolates (36.1%) harbored genes encoding for staphylococcal enterotoxins, including 17/59 (28.8%) isolates from cows and 9/13 (69.2%) from breeders. Moreover, 49.1% and 92.3% of isolates from cows and breeders, respectively, showed penicillin resistance. All isolates were considered as methicillin-susceptible (MSSA). Forty-five (76.3%) of the isolates from cows were slime producers and 52 (88.1%) of them had the ability to form biofilm in microtiter plates. Evidence of a possible zoonotic transmission was observed in two farms, since S . aureus isolates recovered in these farms from cows and breeders belonged to the same clonal lineage (CC15-ST15-t084 or CC30-ST34-t2228). Conclusions Although healthy cows in this study did not harbor methicillin-resistant S . aureus isolates, the nares of healthy cows could be a reservoir of enterotoxigenic and biofilm producing isolates which could have implications in human and animal health. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38849892; PUBMED: 38849892; PUBMEDCENTRAL: PMC11157847 |
| DOI: | 10.1186/s12917-024-04103-x |
| الاتاحة: | https://hal.science/hal-04607821 https://hal.science/hal-04607821v1/document https://hal.science/hal-04607821v1/file/s12917-024-04103-x.pdf https://doi.org/10.1186/s12917-024-04103-x |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.98C89B01 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12917-024-04103-x |
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