التفاصيل البيبلوغرافية
| العنوان: |
Identification and Sequence Analysis of A Family 18 Chitinase-encoding-gene (ChiB) From A Chitinolytic Bacterium Isolated From the Central Highland Region |
| المؤلفون: |
Bich Thuy Vu, Thi Anh Do, Thi Huyen Nguyen, To Uyen Huynh, Tu Oanh Do, Iuliia Pentekhina, Anh Dzung Nguyen, Dinh Minh Tran |
| المصدر: |
The University of Danang - Journal of Science and Technology |
| بيانات النشر: |
University of Da Nang |
| سنة النشر: |
2021 |
| المجموعة: |
neliti (Indonesia's Think Tank Database) |
| مصطلحات موضوعية: |
chitinase, chiB gene, sequence analysis, CBM50, enzyme chitinase, gen chiB, phân tích trình tự, Indonesia |
| الوصف: |
Chitinolytic bacteria and their chitinases have attracted great attention due to potential applications in various fields, including medicine, food processing, agriculture. To develop a novel type of biocontrol agents alternative chemical agents for phytopathogenic controlling, we focus on bacteria possessed high chitinase activity. In this study, a chitinase gene (chiB) from Bacillus velezensis RB.IBE29 was identified, cloned, and analyzed. The ORF of chiB consists of 1,263 base pairs and encodes a deduced protein (BvChiB) of 420 amino acids with a predicted molecular mass of 47.59 kDa. The primary structure analysis of BvChiB revealed that the deduced enzyme is composed of two carbohydrate-binding module family 50 domains at the N-terminus and a catalytic domain at the C-terminus. BvChiB was grouped into subfamily A of bacterial GH18 chitinases based on phylogenetic analysis. Analyses based on the primary and three-dimensional structures showed that differences of important residues were observed between BvChiB and well-known chitinases reported. These analyses imply that BvChiB possibly possesses an interesting role in the degradation of insoluble chitin. This is the first report describing sequence analyses of the chitinase gene from the bacterium. We are conducting the expression, purification, and characterization of BvChiB concerning chitinase and antifungal activities. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
Indonesian |
| Relation: |
https://www.neliti.com/publications/453325/identification-and-sequence-analysis-of-a-family-18-chitinase-encoding-gene-chib |
| الاتاحة: |
https://www.neliti.com/publications/453325/identification-and-sequence-analysis-of-a-family-18-chitinase-encoding-gene-chib |
| Rights: |
(c) The University of Danang - Journal of Science and Technology, 2021 |
| رقم الانضمام: |
edsbas.98ACB12A |
| قاعدة البيانات: |
BASE |